天津中医药2025，Vol.42Issue(3)：353-361,9.DOI:10.11656/j.issn.1672-1519.2025.03.14
马齿苋多糖调节TLR4/NF-κB信号通路对大鼠溃疡性结肠炎的影响
Effect of portulaca oleracea polysaccharide on ulcerative colitis in rats by regulating TLR4/NF-κB signaling pathway
摘要
Abstract
[Objective]To investigate the effect of portulaca oleracea polysaccharides(POP)on ulcerative colitis(UC)in rats by regulating toll type receptor 4(TLR4)/nuclear transcription factor-KB(NF-κB)signaling pathway.[Methods]The 40 rats were randomly divided into normal group,model group,POP group,POP+TLR4 inhibitor resatorvid(TAK-242)group and POP+TLR4 agonist lipopolysaccharide(LPS)group,with 8 rats in each group.The UC rat models were prepared by continuously drinking 5％sodium gluconate sulfate solution for 7 days.And the rats were treated by gavage for 2 weeks.The rats general status were observed and the disease activity index(DAI)scores were measured.The colon tissue samples were taken and measured its length,and then performed the mucosal injury(CMDI)scoring.The pathological changes and cell apoptosis of colon tissue was observed through hematoxylin-eosin(HE)or TdT-mediated dUTP nick end labeling(TUN EL)staining.The level of inflammatory factors[interferon(IFN)-γ,tumor necrosis factor(TNF)-α,interleukin(IL)-1 β,IL-10,myeloperoxidase(MPO)]and oxidative stress indicators[the level of reactive oxygen species(ROS),malondialdehyde(MDA)and activity of superoxide dismutase(SOD),catalase(CAT)]in colon tissue were detected by enzyme linked immunosorbent assay(ELISA).The expression of TLR4,NF-κB p65 and apoptosis related protein and mRNA in colon tissue were detected by Western blot or real-time fluorescence quantitative polymerase chain reaction(RT-PCR)method.[Results]Compared with model group,the general state of the rats in POP group and POP+TAK-242 group were significantly improved.The DAI score,CMDI score and the level of IFN-γ,TNF-α,IL-1β,MPO,ROS,MDA were decreased(P＜0.05);the colon length,IL-10 level and the activity of SOD,CAT were increased(P＜0.05).The pathological changes and cell apoptosis of colon tissue were significantly improved,and the apoptosis index was decreased(P＜0.05).The protein and mRNA expression of TLR4,NF-κB p65,B lymphomatoma-2(Bcl-2)related X protein(Bax),Cleared Caspase-3 in colon tissue were decrease,while the protein and mRNA expression of Bcl-2 was increase(P＜0.05).Compared with POP group,the effect of POP+TAK-242 group on various indicators of UC rats were significantly enhanced(P＜0.05);the effect of POP+LPS group on various indicators were significantly weakened(P＜0.05).[Conclusion]POP has the effect of alleviating colon tissue damage in UC rats,which may be related to downregulating the TLR4/NF-κB signaling pathway,inhibiting inflammation,oxidative stress and cell apoptosis of colon tissue.关键词
溃疡性结肠炎/马齿苋多糖/炎症/氧化应激/凋亡/TLR4/NF-κB信号通路Key words
ulcerative colitis/portulaca oleracea polysaccharides/inflammation/oxidative stress/apoptosis/TLR4/NF-κB signaling pathway分类
医药卫生引用本文复制引用
康维娜,李学永,路新卿..马齿苋多糖调节TLR4/NF-κB信号通路对大鼠溃疡性结肠炎的影响[J].天津中医药,2025,42(3):353-361,9.基金项目
河北省2022年度医学科学研究课题计划(20220491). （20220491）
