天津中医药2025，Vol.42Issue(3)：381-386,6.DOI:10.11656/j.issn.1672-1519.2025.03.17
基于Nrf2/HO-1信号通路探讨三黄益肾胶囊对糖尿病肾病大鼠氧化应激的影响
Effect of Sanhuang Yishen Capsule on oxidative stress in rats with diabetic nephropathy based on Nrf2/HO-1 signaling pathway
摘要
Abstract
[Objective]To study the therapeutic effect of Sanhuang Yishen Capsule on diabetic nephropathy(DKD)model rats and its effect on Nrf2/HO-1 signaling pathway.[Methods]Forty male SD rats of SPF grade were selected.Eight were randomly chosen as normal control group,and the rest of the rats were injected with high-fat chow and streptozotocin(STZ)to establish the DKD model rats,which were randomly divided into the model group,the Sanhuang Yishen Capsule low(0.81g/kg)and high(1.62 g/kg)dose group.The positive group was given Irbesartan(30 mg/kg)by gavage,while the normal group and the model group were given equal volume of distilled water by gavage for four weeks.Firstly,the therapeutic effect of Sanhuang Yishen Capsule on DKD model rats was evaluated by examining the relevant biochemical indexes and renal histopathological changes in the serum and urine of rats in each group after modeling.Secondly,the levels of antioxidant-related indexes of superoxide dismutase(SOD),malondialdehyde(MDA),and glutathione peroxidase(GSH-PX)were detected in the renal tissues of the rats in each group,so as to clarify the therapeutic effect of Sanhuang Yishen Capsule on DKD model rats.Meanwhile the levels of interleukin(IL)-1[3,IL-6 and tumor necrosis factor α(TNF-α)in the homogenates of renal tissues of rats in each group were detected by ELISA to clarify the effect of Sanhuang Yishen Capsule on the inflammatory response of renal tissues of rats in the DKD model.Finally,qPCR and Western blot were used to detect the levels of Nrf2 and HO-1 genes and proteins,which are key factors of Nrf2/HO-1 signaling pathway,in the renal tissues of rats in each group,so as to preliminarily investigate the mechanism of inhibition of oxidative stress by Sanhuang Yishen Capsule in the renal tissues of rats in the DKD model.[Results]Sanhuang Yishen Capsule could significantly reduce the levels of Cr and BUN in the serum of DKD model rats,reduce the 24 h urinary protein(UTP)level,and improve the focal degeneration of renal tubules,glomerular basal pathological changes such as membrane thickening simultaneously.In addition,Sanhuang Yishen Capsule can significantly increase the activities of SOD and GSH-PX in the kidney tissue of DKD model rats,reduce the level of MDA,and reduce the levels of 1L-6,IL-1 β and TNF-α in the serum of DKD rats.The results of qPCR and Western blot showed that Sanhuang Yishen Capsule could significantly up-regulate the gene and protein levels of Nrf2 and HO-1 in the kidney tissue of DKD model rats.[Conclusion]Sanhuang Yishen Capsule has a therapeutic effect on DKD model rats,and its mechanism may be related to up-regulating Nrf2/HO-1 signaling pathway in renal tissue and improving its oxidative stress.关键词
三黄益肾胶囊/糖尿病肾病/氧化应激/Nrf2/HO-1信号通路Key words
Sanhuang Yishen Capsule/diabetic kidney disease/oxidative stress/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
范立荣,边育红,吕树泉,李松林,李函舟,张辉,武锐,刘珍,苏秀海,李建秀,王亚男..基于Nrf2/HO-1信号通路探讨三黄益肾胶囊对糖尿病肾病大鼠氧化应激的影响[J].天津中医药,2025,42(3):381-386,6.基金项目
河北省中医药管理局科研计划项目(2024485) （2024485）
河北省自然科学基金中医药联合基金培育项目(H2022110019) （H2022110019）
苏秀海名老中医工作室. （）
