新医学2025，Vol.56Issue(2)：131-138,8.DOI:10.12464/j.issn.0253-9802.2024-0509
胸腺素β10基因在甲状腺乳头状癌进展中的作用机制
Mechanism of thymosin β10 gene in the progression of papillary thyroid carcinoma
摘要
Abstract
Objective To investigate the mechanism of thymosin β10 gene(TMSB10)promoting the progression of papillary thyroid carcinoma(PTC).Methods The Gene Expression Profiling Interactive Analysis(GEPIA)database was used to analyze highly expressed genes in PTC and their expression levels across different T and N stages.Real-time quantitative PCR(qRT-PCR)was employed to detect the differential expression of TMSB10 in the human thyroid follicular epithelial cell line Nthy-ori 3-1 and PTC cell lines(BCPAP,TPC-1,KTC-1,BHT101).Western blot was used to measure the expression differences of TMSB10 protein in each group.Short hairpin(sh)RNA sequences(sh-TMSB10-NC,sh-TMSB10-1,sh-TMSB10-2 vectors)were constructed in 293T cells and transfected into TPC-1 and BCPAP cells to knock down TMSB10 expression,which was validated by qRT-PCR.Cell proliferation was assessed using the Cell Counting Kit-8(CCK-8)assay,and cell invasion ability was measured using the Transwell assay.Results GEPIA database analysis revealed that TMSB10 was highly expressed in PTC,with statistically significant differences across different T and N stages(all P<0.05).Compared to Nthy-ori 3-1 cells,TMSB10 was highly expressed in PTC cell lines,with statistically significant differences(all P<0.05).The proliferation and invasion abilities of the sh-TMSB10-1 and sh-TMSB10-2 groups were significantly reduced compared to the sh-TMSB10-nc group(P<0.05).Conclusions TMSB10 is highly expressed in PTC,which enhances the proliferation and invasion abilities of PTC cells,promoting its progression.关键词
胸腺素β10基因/甲状腺乳头状癌/侵袭/增殖/作用机制Key words
Thymosin β10 gene/Papillary thyroid carcinoma/Invasion/Proliferation/Mechanism引用本文复制引用
蒙建鑫,金义宽,李雅婷,周许敏,廖方雨,姚繁,李强..胸腺素β10基因在甲状腺乳头状癌进展中的作用机制[J].新医学,2025,56(2):131-138,8.基金项目
广东省医学科研基金(A2023117) （A2023117）
南方医科大学珠江医院院长基金-基础研究培育项目(yzjj2022qn22) （yzjj2022qn22）
