烟草科技2025，Vol.58Issue(3)：41-48,69,9.
苜蓿花叶病毒和白三叶草花叶病毒复合侵染对黄花烟草中3种酚类物质的影响
Effects of co-infection with alfalfa mosaic virus and white clover mosaic virus on three phenolic substances in Nicotiana rustica
摘要
Abstract
To clarify the effect of co-infection with alfalfa mosaic virus(AMV)and white clover mosaic virus(WCMV)on three phenolic substances(chlorogenic acid,total phenols and flavonoids)in Nicotiana rustica,N.rustica inoculated with AMV and WCMV separately and combined in a ratio of 3∶1 was set as the treatment,while N.rustica inoculated with PBS buffer alone served as the negative control.Symptoms of N.rustica were observed and DAS-ELISA was performed 6,18 and 30 days after inoculation.At the same time,the leaves of N.rustica were collected 3,6,9,12,15,18,21,24,27 and 30 days after inoculation with viruses to determine the contents(mass fractions)of chlorogenic acid,total phenols and flavonoids.The results showed that the symptoms of N.rustica after co-infection with the two viruses were more severe than after single infection,showing plant dwarfism,leaf shrinkage and chlorosis.The average reproduction rate after co-inoculation with AMV and WCMV(30.68%and 28.24%)was lower than that after single inoculations(32.19%and 30.27%).After co-inoculation with AMV and WCMV,the contents of chlorogenic acid,total phenols and flavonoids in leaves of N.rustica infected were significantly higher than those inoculated with the single virus.The contents of the three substances reached maximum values of 558.98,177.52 and 33.98 µg·g-1 FW at 30,27 and 30 d after inoculation,respectively.The maximum contents of chlorogenic acid,total phenolic and flavonoids in leaves of N.rustica after co-infection increased by 98.28%,85.90%and 107.76%,103.73%,42.78%and 137.25%,39.03%,40.93%and 63.84%compared to single infection with AMV,WCMV and CK and the differences were significant.The results showed that the production of chlorogenic acid,total phenols and flavonoids in N.rustica after co-infection with the two viruses was correlated with the co-infection stress of AMV and WCMV.关键词
黄花烟草/AMV/WCMV/复合侵染/酚类物质Key words
Nicotiana rustica/Alfalfa mosaic virus(AMV)/White clover mosaic virus(WCMV)/Co-infection/Phenolic substance分类
植物保护学引用本文复制引用
周鑫,梁巧兰,魏列新,陈应娥,赖诗雨,芮静..苜蓿花叶病毒和白三叶草花叶病毒复合侵染对黄花烟草中3种酚类物质的影响[J].烟草科技,2025,58(3):41-48,69,9.基金项目
国家自然科学基金"基于苜蓿花叶病毒和白三叶草花叶病毒复合侵染苜蓿的协生作用及其机理研究"(31860039). （31860039）
