烟草科技2025，Vol.58Issue(3)：49-59,11.
烟草野火病叶际微生物群落结构多样性与碳源代谢表征
Characterization of diversity and carbon metabolism of phyllosphere microbial community in tobacco affected by wildfire disease
摘要
Abstract
To clarify the characteristics of leaf structure and phyllosphere microbial morphology,carbon source metabolic capacity,and community structure diversity for tobacco leaves affected by wildfire disease,scanning electron microscopy,BIOLOG ECO,and Illumina NovaSeq high-throughput sequencing technologies were used to investigate the differences in leaf structure,phyllosphere bacteria and fungal communities between infected and healthy tobacco leaves.The results showed that a significant number of bacterial hyphae,fungal hyphae,and spores adhered to the surfaces of infected tobacco leaves that were severely damaged.The phyllosphere microbial communities of the infected tobacco leaves had the highest utilization capacity for carboxylic acids(methyl pyruvate and D-malate,etc.),whereas those of healthy tobacco leaves had the highest utilization capacity for polymers(Tween-40 and Tween-80,etc.).Both leaf groups were inefficient in the utilization of phenolic compounds(2-hydroxybenzoic acid and 4-hydroxybenzoic acid).Although no significant differences were observed in the phyllosphere bacterial and fungal communities between the infected and healthy tobacco leaves at phylum level,significant differences were observed at the genus level.Dominant bacterial genera of the infected tobacco leaves were Pseudomonas(67.43%)and Pantoea(5.75%),whereas Ralstonia(17.62%),Pseudomonas(15.79%)and Ruminobacter(12.76%)dominated in the healthy samples.Dominant fungal genera were Phoma(24.68%),Alternaria(22.87%)and Phialocephala(5.72%)on the infected tobacco leaves,whereas Cladosporium(14.80%),Sampaiozyma(8.19%)and Mortierella(5.23%)on the healthy tobacco leaves.Significant differences in diversity and richness between phyllosphere bacterial and fungal communities of the infected and healthy tobacco leaves indicated that a composite infection of bacteria such as Pseudomonas spp.and fungi such as Phoma spp.contributed to the progression of tobacco wildfire disease.关键词
烟草野火病/碳源代谢/高通量测序/叶际微生物/群落结构Key words
Tobacco wildfire disease/Carbon source metabolism/High-throughput sequencing/Phyllosphere microorganism/Community structure分类
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童庭,李珍,胡静荣,蔡刘体,陈丽莉,陈兴江,史彩华,汪汉成..烟草野火病叶际微生物群落结构多样性与碳源代谢表征[J].烟草科技,2025,58(3):49-59,11.基金项目
国家自然科学基金项目"烟草关键生育期叶际微生物群落结构及其时空分布格局和代谢功能研究"(32160522) （32160522）
中国烟草总公司科技项目"基于叶际微生态的烟区多病害复合侵染机制研究"[110202101048(LS-08)] （LS-08）
贵州省人才项目"贵州省'百层次'创新型人才"(GCC[2022]028-1、GCC[2023]108) （GCC[2022]028-1、GCC[2023]108）
贵州省科技创新人才团队项目"茄科叶用经济作物病害绿色防控科技创新人才团队建设"(黔科合平台人才-CXTD[2023]021) （黔科合平台人才-CXTD[2023]021）
贵州省烟草公司科技项目"烟草叶部病害高效生防菌发酵关键技术研究与应用"(2024XM06). （2024XM06）
