烟草科技2025，Vol.58Issue(3)：60-69,10.
普通烟草NtWRKY40基因克隆及其抗旱功能
Cloning of NtWRKY40 gene in Nicotiana tabacum and analysis of its drought resistance functions
摘要
Abstract
The NtWRKY40 gene encoding a WRKY transcription factor was cloned from tobacco cv.K326 and the gene was subcellularly mapped and overexpressed in tobacco.By comparing the drought resistance of transgenic and control(wild-type)plants,the physiological indexes of drought resistance and the expression of drought stress response genes were determined,and the drought resistance function of the NtWRKY40 gene was analyzed.The results showed that the coding sequence(CDS)of the tobacco NtWRKY40 gene was 777 bp in length,encoding 258 amino acids.Subcellular localization showed that the gene was mainly expressed in the nucleus.Compared with the control plants,the overexpressed transgenic plants of the NtWRKY40 gene exhibited significantly higher drought resistance,the free proline(Pro)content and the superoxide dismutase(SOD)and peroxidase(POD)activities in the transgenic plants were higher,while the malondialdehyde(MDA)content was lower;and the expression levels of NtSOD,NtAPX,NtP5CS and NtNCED1 in transgenic plants were significantly higher under polyethylene glycol(PEG)-simulated drought stress.This study provided preliminary evidence that the NtWRKY40 gene in tobacco could effectively improve drought resistance and provides a research basis for the application of this gene in plant stress resistance genetic engineering.关键词
普通烟草/NtWRKY40基因/亚细胞定位/干旱胁迫Key words
Nicotiana tabacum/NtWRKY40 gene/Subcellular localization/Drought stress分类
农业科学引用本文复制引用
耿少武,李晓燕,周贵贤,贺丽,刘正杰,柴云霞,丁以纾,林云红,布云虹,王磊,熊茜,艾可彪,孙嘉贤..普通烟草NtWRKY40基因克隆及其抗旱功能[J].烟草科技,2025,58(3):60-69,10.基金项目
中国烟草总公司云南省公司科技重大项目"楚雄K326植烟土壤精准管理及烟叶品质调控关键技术研究"(2023530000241019). （2023530000241019）
