中国肺癌杂志2025，Vol.28Issue(2)：114-130,17.DOI:10.3779/j.issn.1009-3419.2025.105.01
FCN3可作为肺鳞癌预后及免疫治疗的潜在生物标志物
FCN3 Can Serve as A Potential Biomarker for Prognosis and Immunotherapy of Lung Squamous Cell Carcinoma
摘要
Abstract
Background and objective Non-small cell lung cancer(NSCLC)is the leading cause of cancer-related deaths worldwide.Lung squamous cell carcinoma(LUSC)is an important pathological subtype of NSCLC.The complex immune escape mechanism limits the effectiveness of immunotherapy.Ficolin-3(FCN3)is a crucial immunomodulatory mol-ecule that regulates immune escape by remodeling the tumor microenvironment.However,the role of FCN3 in LUSC remains unclear.This study employed bioinformatics methods to analyze LUSC samples from The Cancer Genome Atlas(TCGA)database.The aim of this study was to explore the potential biological functions and prognostic significance of FCN3 in LUSC.Methods A pan-cancer analysis characterized the expression patterns and prognostic value of FCN3 across various cancer types.Simultaneously,the expression patterns of FCN3 in LUSC samples from the TCGA database and its relationship with prognosis were analyzed.The Nomogram model and somatic mutation analysis,differential expression analysis,correlation analysis,as well as Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG),and Gene Set Enrichment Analysis(GSEA)were constructed to explore the potential mechanisms of FCN3.Additionally,immune infiltration analysis,immune escape score (TIDE),and correlation analysis of immune-related molecules were used to reveal the regulatory role of high FCN3 levels on immunity in LUSC. Furthermore,the correlation between FCN3 expression characteristics and drug sen-sitivity was evaluated. Finally,in vitro experiments verified the expression characteristics of FCN3 in LUSC. Results The ex-pression level of FCN3 in LUSC tissues was significantly lower than that in normal tissues. Patients with high FCN3 expression in LUSC had a poorer prognosis compared to those with low expression. Different expression levels of FCN3 were associated with the abundance of immune cell infiltration and immune cell dysfunction. It was also linked to the expression of immune checkpoints,immune stimulatory molecules,major histocompatibility complex (MHC) class molecules,and chemotherapy drug sensitivity. Conclusion High expression of FCN3 in LUSC is associated with poor prognosis and is linked to immune cell infiltration,immune-related pathways,and immune-related molecules. FCN3 may be a potential prognostic marker and a new target for immunotherapy in LUSC.关键词
FCN3/肺肿瘤/免疫/生物标志物Key words
FCN3/Lung neoplasms/Immunity/Biomarker引用本文复制引用
李伟,祖玲玲,徐嵩..FCN3可作为肺鳞癌预后及免疫治疗的潜在生物标志物[J].中国肺癌杂志,2025,28(2):114-130,17.基金项目
本研究受国家自然科学基金项目(No.82172776)、天津市卫生健康科研项目(No.TJWJ2023XK005)和天津市科技计划项目(No.24ZXGZSY00010)资助 This study was supported by the grants from National Natural Science Foundation of China(No.82172776),Tianjin Health Research Project(No.TJWJ2023XK005)and Tianjin Science and Technology Plan Project(No.24ZXGZSY00010)(All to Song XU). （No.82172776）
