中国水产科学2025，Vol.32Issue(1)：60-73,14.DOI:10.12264/JFSC2024-0204
基于环境DNA技术的夏、秋季刺参养殖池塘浮游植物群落结构研究
Phytoplankton community structure of Apostichopus japonicus culti-vation ponds based on environmental DNA technology in summer and autumn
摘要
Abstract
Environmental DNA(eDNA)technology was applied to monitor six Apostichopus japonicus cultivation ponds during the summer and autumn of 2023 to investigate the phytoplankton community structure and its impact on the environment in sea cucumber(Apostichopus japonicus)cultivation ponds.The survey results showed that 8 phyla,103 genera,and 162 species of phytoplankton were detected in the summer,while 8 phyla,248 genera,and 465 species were recorded in the autumn.The phytoplankton species composition and relative abundance were dominated by Dinoflagellata,Chlorophyta,and Bacillariophyta.A seasonal succession was observed in dominant species,with 41 dominant species identified,mainly belonging to Dinoflagellata,Chlorophyta,and Bacillario-phyta.The biodiversity and evenness indices of phytoplankton were lower in summer than in autumn,whereas the richness index was higher in summer.According to biodiversity indices,the A.japonicus cultivation ponds were classified as lightly to moderately polluted during the study period.Redundancy analysis(RDA)of the phytoplan-kton community structure and environmental factors revealed that the relative abundances of Bacillariophyta,Dinoflagellata,and Chlorophyta were positively correlated with nitrate concentration,phosphate concentration,and total phosphorus,nitrate,and ammonia nitrogen,respectively.These findings provided a scientific basis for environmental regulation in A.japonicus cultivation.关键词
浮游植物群落/刺参/环境因子/环境DNA技术Key words
phytoplankton community/Apostichopus japonicus/environmental factors/environmental DNA technology分类
农业科技引用本文复制引用
阮述超,常亚青,刘子毓,叶琪,韩泠姝,肖皓冉,丁君,王荦,赵冲,尹东红..基于环境DNA技术的夏、秋季刺参养殖池塘浮游植物群落结构研究[J].中国水产科学,2025,32(1):60-73,14.基金项目
辽宁省"兴辽英才计划"领军人才项目(XLYC2202001) （XLYC2202001）
辽宁省科学技术厅农业重大专项(2023JH1/10200007) （2023JH1/10200007）
大连市高层次人才支持项目(2020RD03). （2020RD03）
