中国比较医学杂志2025，Vol.35Issue(1)：30-40,11.DOI:10.3969/j.issn.1671-7856.2025.01.003
柯萨奇病毒A组6型和B组1型混合感染叙利亚金黄地鼠动物模型的实验研究
Coinfection with coxsackievirus A6 and B1 in a Syrian hamster animal model
摘要
Abstract
Objective To establish an animal model of hand,foot,and mouth disease(HFMD)in Syrian hamsters coinfected with coxsackievirus A6(CVA6)and coxsackievirus B1(CVB1).Methods 42 Syrian hamsters were divided into a CVA6 infection group,CVB1 infection group,CVA6 and CVB1 coinfection group and control group.A HFMD model was established by nasal instillation of virus solution and phosphate-buffered saline.Clinical and physiological indicators and detoxification status were monitored and recorded for 15 d,and animals were selected on day 7(D7)after infection for histopathology and viral antigen and nucleic acid testing.Results Hamsters in the single-infection and coinfection groups showed clinical symptoms similar to human HFMD.White blood cell,neutrophil,and lymphocyte result were characteristic of viral infection.Both viral nucleic acids were detected in throat swabs,feces,blood,and tissues and both viruses were isolated from fecal samples.Pathological damage and positive co-localization of CVA6 and CVB1 viral antigen proteins and nucleic acids were found in brain and other tissues.Conclusions Nasal instillation of a CVA6 and CVB1 mixture can successfully coinfect Syrian hamsters,replicate herpes infection similar to human HFMD,and cause pathological viral myocarditis and encephalitis damage.The result showed that the coinfection group was more seriously affected than the single-infection group,with worse clinical symptoms,increased viral replication,and obvious tissue pathological damage.This study provides a reference for further basic and clinical research into human enterovirus coinfection.关键词
叙利亚金黄地鼠/混合感染/动物模型/柯萨奇病毒A组6型/柯萨奇病毒B组1型/手足口病Key words
Syrian hamster/coinfection/animal model/coxsackievirus A6/coxsackievirus B1/hand,foot and mouth disease引用本文复制引用
侯婧涵,赵远,杨凤梅,和占龙,段素琴,徐鸿界,孙文亭,李明学,李艳艳,靳玮华,陈丽雄,刘权..柯萨奇病毒A组6型和B组1型混合感染叙利亚金黄地鼠动物模型的实验研究[J].中国比较医学杂志,2025,35(1):30-40,11.基金项目
北京协和医学院中央高校基本科研业务费(3332023079) （3332023079）
云南省基础研究专项-面上项目(202401CF070048) （202401CF070048）
中国医学科学院医学生物学研究所所级重点科研专项(2022IMBCAMS002). （2022IMBCAMS002）
