中国比较医学杂志2025，Vol.35Issue(1)：41-48,8.DOI:10.3969/j.issn.1671-7856.2025.01.004
香菇多糖抑制TNFα-铁自噬拮抗亚砷酸钠染毒小鼠肝组织铁死亡
Lentinan inhibits tumor necrosis factor α-ferritinophagy and antagonizes hepatic tissue ferroptosis in sodium arsenite-exposed mice
摘要
Abstract
Objective To explore the effect and mechanism of lentinan(LNT)on hepatic tissue ferroptosis in mice exposed to sodium arsenite(SA).Methods C57BL/6N male mice were exposed to SA low-dose,SA high-dose,and LNT intervention combined with SA high-dose,then,hematoxylin and eosin(HE)staining was applied to assess pathological liver tissue damage;Enzyme-linked immunosorbent and Western blot were used to detect the content or expression of tumor necrosis factor α(TNFα),interleukin-6(IL-6),ferritinophagy or ferroptosis biomarkers.Results Compared with the control group,SA exposure induced the elevated levels of TNFα,IL-6,ferritinophagy biomarker ferritin heavy chain 1(FTH1)and microtubule-associated protein 1 light chain 3B(MAP1LC3B)in mice liver tissue,while levels the ferroptosis biomarker GPX4 decreased(P＜0.05).Compared with SA high-dose groups,LNT intervention showed the reduced pathological liver damage and the downregulated levels of TNFα,IL-6,FTH1,and MAP1LC3B,while the level of GPX4 upregulated(P＜0.05).Western blot experiment showed that LNT intervention antagonized the upregulated levels of FTH1,and autophagy biomarker LC3B/A,and antagonized the increased co-expressions of FTH1 with LC3B or Ub protein in SA high-dose group(P＜0.05).Conclusions LNT antagonizes SA-exposed hepatic pathological injury and ferroptosis in mice,possibly associated with inhibition of TNFα-ferritinophagy signaling.关键词
亚砷酸钠/肝毒性/铁死亡/香菇多糖/铁自噬Key words
sodium arsenite/hepatotoxicity/ferroptosis/lentinan/ferritinophagy引用本文复制引用
杨渊,鲍家成,邓业康,陈秧,何琴..香菇多糖抑制TNFα-铁自噬拮抗亚砷酸钠染毒小鼠肝组织铁死亡[J].中国比较医学杂志,2025,35(1):41-48,8.基金项目
广西自然科学基金(2023JJA140042) （2023JJA140042）
湖南省自然科学基金(2021JJ30484,2022JJ30426) （2021JJ30484,2022JJ30426）
广西大学生创新训练项目(202410601041). （202410601041）
