中国比较医学杂志2025，Vol.35Issue(1)：79-85,7.DOI:10.3969/j.issn.1671-7856.2025.01.008
5-Aza-CdR通过抑制大鼠原代肾肌成纤维细胞Epo基因启动子高甲基化逆转PMT
5-Aza-CdR reverses pericyte-myofibroblast transition by inhibiting Epo gene promoter hypermethylation in rat primary renal myofibroblasts
摘要
Abstract
Objective To examine the effect of the demethylating agent 5-aza-2'-deoxycytidine(5-Aza-CdR)on pericyte-myofibroblast transition(PMT)in primary rat renal myofibroblasts.Methods Rat primary renal myofibroblasts were treated with 5-Aza-CdR 250 ng/mL for 72 h,and the degree of Epo promoter methylation was detected by pyrosequencing.Protein expression levels of α-smooth muscle actin(α-SMA),platelet-derived growth factor receptor-β(PDGFRβ)and DNA methyltransferase(Dnmt3a)were detected by immunofluorescence and Western blot,and erythropoietin(EPO)levels in the supernatant were detected.Results Compared with the control group,5-Aza-CdR treatment significantly decreased the expression of Dnmt3a and hypermethylation of the Epo promoter,and subsequently decreased the expression of α-SMA and the expression ratio of α-SMA to PDGFRβ in myofibroblasts.Meanwhile,5-Aza-CdR treatment increased the level of EPO in the cell supernatant.Conclusions 5-Aza-CdR can reverse PMT by inhibiting Epo promoter hypermethylation in primary renal myofibroblasts.关键词
周细胞-肌成纤维细胞转化/EPO/DNA甲基化/5-氮杂-2'脱氧胞苷/DNA甲基转移酶Key words
pericyte-myofibroblast transition/EPO/DNA methylation/5-aza-2'-deoxycytidine/DNA methyltransferase引用本文复制引用
袁玲,王蕾,程鹏,江茜,崔晓雪..5-Aza-CdR通过抑制大鼠原代肾肌成纤维细胞Epo基因启动子高甲基化逆转PMT[J].中国比较医学杂志,2025,35(1):79-85,7.基金项目
天津市卫生健康科技项目(TJWJ2022MS050). （TJWJ2022MS050）
