中国兽医科学2025，Vol.55Issue(3)：285-290,6.DOI:10.16656/j.issn.1673-4696.2025.0036
非洲猪瘟病毒R298L基因缺失毒株的构建及其致病性与免疫原性评价
Construction and evaluation of pathogenicity and immunogenicity of African swine fever virus R298L gene deletion strain
摘要
Abstract
To explore the function of African swine fever virus(ASFV)R298L gene,elucidate the pathogenic mechanism of ASFV,and provide a candidate strain for the vaccine research,the gene deletion ASFV-△R298L was constructed by homologous recombination.The pathogenicity and immune response of ASFV-△R298L and ASFV CN/GS/2018 were evaluated in pigs.The results showed that animals infected with ASFV-WT had severe fever and all died within 13 days after infection,which severe lesions occurred in multiple organs,but the animals infected with ASFV-△R298L had no fever or lesions in organs and all survived during the observation period.More importantly,pigs infected with ASFV-△R298L produced high levels of neutralizing antibody in late stage.In summary,these findings provide insights into analysis the pathogenic mechanism of ASFV,and provide important ideas for the development of gene deletion candidate vaccines.关键词
非洲猪瘟病毒/R298L基因/缺失毒株/致病性/免疫保护Key words
African swine fever virus/R298Lgene/deletion strain/pathogenicity/immunoprotection分类
农业科技引用本文复制引用
范许许,朱紫祥,李伟伟,朱兆宇,裴丹诗,王一卓,何路,李熙忠,任青峰,郑海学..非洲猪瘟病毒R298L基因缺失毒株的构建及其致病性与免疫原性评价[J].中国兽医科学,2025,55(3):285-290,6.基金项目
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2024KJ14) （2024KJ14）
国家重点研发计划项目(2021YFD1800300) （2021YFD1800300）
中央高校基本科研业务费专项(lzujbky-2022-ct02) （lzujbky-2022-ct02）
甘肃省科技重大专项(22ZD6NA001,22ZD6NA012) （22ZD6NA001,22ZD6NA012）
国家自然科学基金青年项目(32202781,32302857) （32202781,32302857）
甘肃省青年科技基金计划项目(22JR5RA034) （22JR5RA034）
中国博士后科学基金项目(2023M743830) （2023M743830）
国家生猪产业技术体系项目(CARS-35,CARS-39-13) （CARS-35,CARS-39-13）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
中国农业科学院创新计划项目(CAAS-CSLPDCP-2023002,CAAS-ASTIP-2024-LVRI) （CAAS-CSLPDCP-2023002,CAAS-ASTIP-2024-LVRI）
甘肃省创新群体项目(23JRRA546) （23JRRA546）
