中国兽医科学2025，Vol.55Issue(3)：291-298,8.DOI:10.16656/j.issn.1673-4696.2025.0030
哺乳动物呼肠孤病毒σ3蛋白对细胞自噬的调控作用
Study on the regulatory effect of mammalian reovirus σ3 protein on autophagy
摘要
Abstract
Indirect immunofluorescence was used to detect the effects of mammalian reovirus(MRV)infection and σ 3 protein on the formation of autophagosomes,and the colocalization of σ3 protein with translocase of outer mitochondrial membrane 20(TOMM20).Western-blotting was used to analyze the ef-fects of MRV infection and σ3 protein on the expression of autophagy markers LC3B,SQSTM1/p62,Beclin 1,ATG5 and adenosine monophosphate-activated protein kinase-mammalian target of rapamycin(AMPK-mTOR)signaling pathway factors AMPK,p-AMPK,mTOR and p-mTOR.The results showed that MRV infection and the presence of σ3 protein could transfer the mCherry-GFP-LC3B fusion protein from the cytoplasm to the autophagosome membrane,promoting the early formation of autophagy.It was also found that MRV infec-tion and σ3 protein could promote the increase of the transition from autophagy marker LC3B-Ⅰ to LC3B-Ⅱ,up-regulate the protein expression of Beclin 1,ATG5,ATG7 and p-AMPK,and down-regulate the protein expressions of p-mTOR and autophagy receptor SQSTM1/p62.This study revealed that σ 3 protein enhances the occurrence of autophagy through the AMPK/mTOR signaling axis,laying a foundation for further elu-cidating the pathogenic mechanism of MRV and providing a scientific basis for the prevention and con-trol of infectious diseases caused by MRV.关键词
细胞自噬/哺乳动物呼肠孤病毒/σ3蛋白Key words
autophagy/mammalian reovirus/σ3 protein分类
畜牧业引用本文复制引用
白辉盛,成荣荣,董平安,李骁昳,莫荣纤,李殿玉,马婷婷,李向茸,冯若飞..哺乳动物呼肠孤病毒σ3蛋白对细胞自噬的调控作用[J].中国兽医科学,2025,55(3):291-298,8.基金项目
西北民族大学大学生创新训练项目(X202310742266) （X202310742266）
西北民族大学中央高校基本科研业务费资金项目(31920240125-01) （31920240125-01）
甘肃省高校教师创新基金项目(2025B-33) （2025B-33）
