塞内加谷病毒VP1蛋白对细胞因子的影响OA北大核心

In order to investigate the effect of Seneca Valley virus(SVV)VP1 on cytokines,this study constructed eukaryotic plasmid expressing VP1 protein through RT-PCR,double enzyme digestion and ligation assay.Western-blot assay was used to identify whether VP1 was correctly expressed,and fluorescence quantification assay was used to detect the effects of VP1 overexpression on cytokines IL-1α,IL-1β,CCL-2,CCL-5,and TNF-α.The double enzyme digestion assay result showed that the construc-tion of VP1 eukaryotic plasmids was successful,named pEGFP-C1-VP1.Fluorescence quantification assay results showed that VP1 protein significantly promoted the transcription of IL-1α at hour 24 post-transfection,significantly promoted and inhibited the transcription of IL-1β at hour 12,24 and 36,significantly promoted the transcription of CCL-2 at hour 12 and 24,significantly promoted the transcription of CCL-5 at hour 12,24 and 36,and significantly promoted the transcription of TNF-α at hour 12 and 24.The results indicate that SVV VP1 protein can induce transcription of IL-1α,CCL-2,CCL-5,and TNF-α;For IL-1β,SVV VP1 protein promotes IL-1β transcription in the early stage and in-hibits IL-1β transcription in the later stage.