中国兽医科学2025，Vol.55Issue(3)：308-313,6.DOI:10.16656/j.issn.1673-4696.2025.0038
塞内加谷病毒VP1蛋白对细胞因子的影响
Effect of porcine Seneca Valley virus VP1 protein on cytokines
摘要
Abstract
In order to investigate the effect of Seneca Valley virus(SVV)VP1 on cytokines,this study constructed eukaryotic plasmid expressing VP1 protein through RT-PCR,double enzyme digestion and ligation assay.Western-blot assay was used to identify whether VP1 was correctly expressed,and fluorescence quantification assay was used to detect the effects of VP1 overexpression on cytokines IL-1α,IL-1β,CCL-2,CCL-5,and TNF-α.The double enzyme digestion assay result showed that the construc-tion of VP1 eukaryotic plasmids was successful,named pEGFP-C1-VP1.Fluorescence quantification assay results showed that VP1 protein significantly promoted the transcription of IL-1α at hour 24 post-transfection,significantly promoted and inhibited the transcription of IL-1β at hour 12,24 and 36,significantly promoted the transcription of CCL-2 at hour 12 and 24,significantly promoted the transcription of CCL-5 at hour 12,24 and 36,and significantly promoted the transcription of TNF-α at hour 12 and 24.The results indicate that SVV VP1 protein can induce transcription of IL-1α,CCL-2,CCL-5,and TNF-α;For IL-1β,SVV VP1 protein promotes IL-1β transcription in the early stage and in-hibits IL-1β transcription in the later stage.关键词
塞内加谷病毒/VP1蛋白/细胞因子/荧光定量Key words
Seneca Valley virus/VP1 protein/cytokine/fluorescent quantitation分类
农业科技引用本文复制引用
董建国,丛锋,陶梦筱,马云欣,刘培研,王莹,杨灿,杨大苗,刘向楠,张宁..塞内加谷病毒VP1蛋白对细胞因子的影响[J].中国兽医科学,2025,55(3):308-313,6.基金项目
国家重点研发计划项目(2021YFF0703300) （2021YFF0703300）
河南省高等学校重点科研项目(25A230010) （25A230010）
信阳农林学院大学生创新创业训练计划项目(2024010) （2024010）
