中国兽医科学2025，Vol.55Issue(3)：346-353,8.DOI:10.16656/j.issn.1673-4696.2025.0042
对五种qPCR和PCR方法在弓形虫诊断中的比较研究
A comparative study of five qPCR and PCR methods for Toxoplasma gondii diagnosis
摘要
Abstract
To evaluate the performance of 5 PCR and real-time quantitative PCR(qPCR)methods involved in 4 domestic and international standards for diagnosing toxoplasmosis,the sensitivity,repeatability,and specificity of each detection method were assessed based on Toxoplasma gondii tachyzoites.Sixty-two cat fecal samples were used to compare the diagnostic methods mentioned above.The results showed that all standard methods have good specificity and repeatability.Among them,the qPCR method in the SN/T,the qPCR method in the WOAH,and the PCR method in the NY/T had the lowest detection limit(approximately 0.375 tachyzoites/μL),fol lowed by the PCR method in the GB/T(approximately 3.75 tachyzoites per microlitre).The PCR method in the SN/T has the lowest sensitivity(approximately 375 tachyzoites per microlitre).Analysis of testing 62 cat fecal samples revealed that the qPCR method in the SN/T had the highest sensitivity(99.23％),the PCR method in the GB/T had the highest specificity(99.13％),the PCR method in the SN/T had the lowest sensitivity(91.73％),and the PCR method in the NY/T had the lowest specificity(96.82％).This study provides a reference for the selection of standard methods for diagnosing toxoplasmosis.关键词
弓形虫/标准/PCR/qPCR/方法评价Key words
Toxoplasma gondii/standards/PCR/real-time quantitative PCR/method evaluation分类
畜牧业引用本文复制引用
黄艳丽,陈亚娜,白邵缘,周国庆,崔灿,李宇渊,黄思扬,原霖..对五种qPCR和PCR方法在弓形虫诊断中的比较研究[J].中国兽医科学,2025,55(3):346-353,8.基金项目
国家重点研发计划项目(2022YFC2304003)和江苏省"双创计划"-双创人才项目 （2022YFC2304003）
