中国药物经济学2025，Vol.20Issue(2)：77-81,5.DOI:10.12010/j.issn.1673-5846.2025.02.015
基于外泌体基因序列构建miRNA-mRNA预测初治和复治肺结核共有靶基因
Construction of miRNA-mRNA based on Gene Sequences in Exosomes Predicts Key Genes Common in Primary and Refractory Pulmonary Tuberculosis
摘要
Abstract
Objective To construct a potential miRNA-mRNA co-regulatory network for treatment-naïve and retreated pulmonary tuberculosis with the help of bioanalysis,and to elucidate the regulatory mechanism of the common target genes for primary treatment and retreatment of pulmonary tuberculosis.Methods Corresponding differentially expressed mirnas and mrnas were obtained according to the established peripheral blood exosome database,and differentially expressed mirnas were screened from ATB and RTB,and potential target genes of differentially expressed mirnas were predicted,and gene ontology(GO)function annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were further conducted.At the same time,the target gene was obtained by the intersection of the target gene and the differentially expressed gene,and finally the protein-protein interaction(PPI)network was constructed and the key genes were screened.Results A total of 19 differentially expressed miRNAs were obtained by intersecting the differentially expressed miRNAs of ATB and RTB.The GO functional annotation focuses on the SRP-dependent co-translation protein targeting membrane,the co-translated protein targeting the membrane,the nuclear transcription of mRNA catabolism,and the ubiquitin protein ligase binding.The enrichment of KEGG pathway was concentrated in non-small cell lung cancer and FoxO signaling pathway.PPI analysis of high-expression miRNA target genes:HSPA8,RPS27A,RPS6,FBL,and low-expression miRNA target genes:RPL35,HSP90AB1,NACA,NCL.Conclusion By constructing a potential miRNA-mRNA regulatory network related to the co-pathogenesis of ATB and RTB,this paper provides a theoretical basis for further study of the molecular mechanism of ATB and RTB co-pathogenesis,and also provides a scientific basis for the development of new therapeutic targets.关键词
初治肺结核/复治肺结核/生物学分析/外泌体/miRNA/mRNAKey words
Initial treatment of tuberculosis/Re-treatment of tuberculosis/Biological analysis/Exosomes/miRNA/mRNA分类
医药卫生引用本文复制引用
徐振华,李奇凤..基于外泌体基因序列构建miRNA-mRNA预测初治和复治肺结核共有靶基因[J].中国药物经济学,2025,20(2):77-81,5.基金项目
2022年度"天山英才"培养计划项目(2022TSYCCX0105) （2022TSYCCX0105）
