中国药物经济学2025，Vol.20Issue(2)：89-92,4.DOI:10.12010/j.issn.1673-5846.2025.02.018
超高效液相色谱法测定贵州党参中党参炔苷含量
Determination of Lobetyolin Content in Guizhou Codonopsis Radix by UPLC
摘要
Abstract
Objective To establish an UPLC method for the determination of lobetyolin in Guizhou Codonopsis Radix and to provide a method reference for the quality control of Guizhou Codonopsis Radix.Methods Waters CORTECS T3 chromatographic column was used.Mobile phase:acetonitrile-water(containing 0.1%phosphoric acid)(23∶77),flow rate:0.4 ml/min;the detection wavelength was 268 nm.Results The linear equation of lobetyolin was Y=3.196 3×104X+3.304 7×103(r=0.999 3).Linear range:0.010 81～1.080 8 µg,the average recovery of lobetyolin was 98.87%(n=6).Conclusion The established UPLC method was used to determine the content of lobetyolin in Guizhou Codonopsis Radix.The operation was fast and simple,the results were accurate,and the repeatability was good.It has strong practicability in practical work and can be used for quality control of Guizhou Codonopsis Radix.关键词
超高效液相色谱法/贵州党参/党参炔苷/质量控制Key words
UPLC/Guizhou Codonopsis Radix/Lobetyolin/Quality control分类
医药卫生引用本文复制引用
路利亚,何羽,雷联伟..超高效液相色谱法测定贵州党参中党参炔苷含量[J].中国药物经济学,2025,20(2):89-92,4.基金项目
毕节市创新平台及人才团队科技计划项目(毕科合[2023]16号) （毕科合[2023]16号）
