中国药物经济学2025，Vol.20Issue(2)：93-98,6.DOI:10.12010/j.issn.1673-5846.2025.02.019
对照提取物在五加皮质量控制中的应用
Application of Control Extracts in Quality Control of Radix Acanthopanax L.
摘要
Abstract
Objective To explore the quality control method of Acanthopanacis Cortex,extract Acanthopanacis Cortex were used as control.Methods The determination by High Performance Liquid Chromatography(HPLC)was performed on Waters AtlantisTM T3 column with column temperature of 35℃.The acetonitrile(A)-0.1%phosphoric acid solution(B)was used as mobile phase by gradient elution.The content of each component in Acanthopanacis Cortex was calculated by external standard method.Results The linear ranges of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,1,3-dicaffeioylquinic acid,3,5-dicaffeioylquinic acid,4,5-dicaffeioylquinic acid,and kaurenic acid were 0.254 4～7.631、2.742～82.26、0.216 5～6.494、0.043 12～1.293、2.327～69.82、2.165～64.94、0.184 0～5.520、4.456～133.7 μg/ml(r≥0.994 2)respectively.The recoveries rate were 92.9%～102.7%(RSD=0.90%～4.31%,n=6).Conclusion The method is simple,rapid and economical,and can be used for content determination of 8 components in Acanthopanacis Cortex.It can be used for quality control of Acanthopanacis Cortex.关键词
五加皮/苯丙素类/二萜类/对照提取物/高效液相色谱法/含量测定/质量控制Key words
Acanthopanacis Cortex/Phenylpropanoids/Diterpenoids/Reference extracts/HPLC/Determination of content/Quality control分类
医药卫生引用本文复制引用
张佳莉,薛平,汪嘉丽,钱怡云,李莉..对照提取物在五加皮质量控制中的应用[J].中国药物经济学,2025,20(2):93-98,6.基金项目
江苏省药品监督管理局2022年科研项目(202244) （202244）
