中国药物经济学2025，Vol.20Issue(2)：99-103,5.DOI:10.12010/j.issn.1673-5846.2025.02.020
醇沉法和超滤法制备灵芝孢子多糖的抗氧化活性比较
Comparison of Antioxidant Activity of Ganoderma lucidum Spore Polysaccharides Prepared by Alcohol Precipitation and Ultrafiltration
摘要
Abstract
Objective To separate and purify Ganoderma lucidum spore polysaccharides(G.lucidum spore polysaccharides)by alcohol precipitation and ultrafiltration,and compare the antioxidant activity of the two kinds of G.lucidum spore polysaccharides.Methods G.lucidum spore polysaccharides was isolated and purified by alcohol precipitation and ultrafiltration,using defatted and wall-broken G.lucidum spore powder as raw material,and the yield and content of G.lucidum spore polysaccharides were determined,the antioxidant activity was analyzed by hydroxyl radical scavenging ability,reduction ability and chelate Fe2+activity.Results The yield and content of polysaccharide from G.lucidum spore polysaccharides prepared by ultrafiltration were higher than those prepared by alcohol precipitation,both G.lucidum spore polysaccharides showed good antioxidant activity,the Fe2+chelating ability of G.lucidum spore polysaccharides by alcohol precipitation was better than that by ultrafiltration.Conclusion Both G.lucidum spore polysaccharides have antioxidant activity,and their antioxidant mechanism may be related to their ability to scavenge free radicals,reduce the activity of oxidants,and inhibit the occurrence of oxidative reactions,the results can provide experimental data for the industrial preparation of G.lucidum spore polysaccharides and its application in anti-oxidation field.关键词
灵芝孢子多糖/醇沉法/超滤法/抗氧化活性Key words
Ganoderma lucidum spore polysaccharides/Alcohol precipitation/Utrafiltration/Antioxidant activity分类
医药卫生引用本文复制引用
王彤,陈晓清,罗爱勤,吴凤,谢文津,梁君豪,邱麟贻,曹颖男,付裕..醇沉法和超滤法制备灵芝孢子多糖的抗氧化活性比较[J].中国药物经济学,2025,20(2):99-103,5.基金项目
2022年广东省科技创新战略专项"攀登计划"专项(pdjh2022a0746) （pdjh2022a0746）
广州新华学院2022年教学质量与改革工程建设项目课程教研室专项(2022JYS002) （2022JYS002）
