中国中药杂志2025，Vol.50Issue(6)：1534-1543,10.DOI:10.19540/j.cnki.cjcmm.20241215.103
膜荚黄芪黄酮糖基转移酶AmGT90的功能表征
Functional characterization of flavonoid glycosyltransferase AmGT90 in Astragalus membranaceus
摘要
Abstract
Astragalus membranaceus(A.membranaceus),a traditional tonic,contains flavonoids as one of its main bioactive components and key indicators for quality standard detection.These compounds predominantly exist in glycosylated forms after glycosylation modification within the plant.The catalytic products of flavonoid glycosyltransferases in A.membranaceus have been reported to be mostly monoglycosides,and only AmUGT28 catalyzes luteolin to form diglycosides.In this study,we cloned a glycosyltransferase gene,AmGT90,from A.membranaceus,with an ORF length of 1 335 bp,encoding 444 amino acids,and the protein had a relative molecular mass of 50.5 kDa.Phylogenetic tree analysis indicated that AmGT90 belongs to the UGT74 family.In vitro enzymatic reaction showed that AmGT90 had broad substrate specificity and could catalyze the glycosylation of various flavonoids,including isoflavones,flavones,flavanones,and chalcones.AmGT90 not only catalyzed the formation of monoglycosides but also diglycosides.In addition,the mechanism of AmGT90 catalyzing the formation of diglycosides from luteolin was preliminarily explored.The experimental results showed that AmGT90 may preferentially recognize C4'-OH of luteolin and then recognize C7-OH to form diglycosides.This study reported a glycosyltransferase from A.membranaceus capable of converting flavonoids into monoglycosides and diglycosides.This finding not only enhances our understanding of the biosynthetic pathways of flavonoid glycosides in A.membranaceus but also introduces a new component for glycoside production through synthetic biology.关键词
膜荚黄芪/糖基转移酶/黄酮/UGT74/功能鉴定Key words
Astragalus membranaceus/glycosyltransferase/flavonoid/UGT74/functional characterization引用本文复制引用
彭国情,徐冰艳,黄建萍,余志银,黄胜雄..膜荚黄芪黄酮糖基转移酶AmGT90的功能表征[J].中国中药杂志,2025,50(6):1534-1543,10.基金项目
国家自然科学基金杰出青年基金项目(82225043) （82225043）
