浙江大学学报（理学版）2025，Vol.52Issue(2)：263-274,12.DOI:10.3785/j.issn.1008-9497.2025.02.010
钱塘江地表水有机微污染物AhR效应分析
Analysis of AhR effect of organic micropollutants in surface water of Qiantang River
摘要
Abstract
The Qiantang River has received the attentions of many researchers.A large number of organic micropollutants have been detected,which may cause physiological,biochemical,and toxicological effects in aquatic organisms and threaten ecological conditions and human health.However,studies on the human-derived aromatic hydrocarbon receptor(AhR)effects of organic micropollutants in surface waters of the Qiantang River are still limited.This study was conducted to explore the pollution status of organic micropollutants in the surface water of Hangzhou section of Qiantang River through target chemistry,in vitro bioassay,and the toxicity contribution of AhR effects based on the iceberg model,in order to assess the toxicity risk of surface water of Qiantang River to AhR effects from human sources.Targeted chemical analysis detected four categories of organic micropollutants:pesticides,phthalates,perfluorinated compounds and polycyclic aromatic hydrocarbons.The results of chemical analysis showed that the average concentration of perfluorinated compounds was 109.52 ng·L 1,which was the highest cumulative concentration of pollutants detected at each site,and its cumulative concentration ranged from 219.02 to 3 645.54 ng·L-1.In particular,perfluorooctanoic acid(PFOA)is the main pollutant found in perfluorinated compounds in this study.In vitro bioassays showed a dose-dependent increase in the level of transcriptional activity of human-derived AhR receptors with strong transcriptional activity.Particularly,the transcriptional activity level of Fuchun River(80 REF)was 21.53 times higher than that of the blank control group,and Puyang River and Qibao had already shown significant AhR effects under the condition of no enrichment.The equivalent concentration range of the AhR effect in surface water extracts from Qiantang River was 17.61～46.17 ng·L-1B[a]P-EQ,which was higher than the limit value of 4.20 ng·L-1B[a]P-EQ for surface water proposed by the European Union Water Framework Directive.Calculation of the toxicity contribution ratio showed that the main contribution was from polycyclic aromatic hydrocarbons(37.03％～88.15％),followed by pesticides(5.08％～14.30％),and phthalates(2.62％～9.21％).Chrysene(Chr)was the major contributor to AhR mediated activity,followed by benzo[a]anthracene(BaA)and benzo[b]fluoranthene(BbF).In conclusion,the data in this article showed that surface water extracts from the Qiantang River significantly activated the AhR effect.This study provides new insights into the toxic effects of mixed pollutants and human health risk assessment.关键词
有机微污染物/基于生物效应的评估方法/芳香烃受体效应/毒性贡献率Key words
organic micropollutants/effect based methods/aromatic hydrocarbon receptor effects/toxicity contribution rate分类
环境科学引用本文复制引用
万奇霖,刘瑶,洪文俊,应林浩,姚佳,叶铖,范思哲,方文迪..钱塘江地表水有机微污染物AhR效应分析[J].浙江大学学报（理学版）,2025,52(2):263-274,12.基金项目
国家自然科学基金资助项目(22106157) （22106157）
辐射防护实验室开放基金项目(ZFYHHJMN-2023003) （ZFYHHJMN-2023003）
浙江省省级高校基本科研业务费项目. （）
