浙江中医药大学学报2025，Vol.49Issue(2)：132-140,9.DOI:10.16466/j.issn1005-5509.2025.02.002
从预测效度角度探讨腺样体肥大动物模型的建立
Discussion on the Experimental Animal Model of Adenoid Hypertrophy from the Perspective of Predictive Validity
摘要
Abstract
[Objective]To evaluate the feasibility of animal model of simulated adenoid hypertrophy by combining animal model of allergic rhinitis and chronic pharyngitis from the perspective of predictive validity.[Methods]Forty SD rats were randomly divided into blank group,model control group,montelukast group and traditional Chinese medicine(TCM)group,with 10 rats in each group.The model control group,montelukast group and TCM group all established rat model of allergic rhinitis and the rat model of chronic pharyngitis was also established in the same time,which combined the simulated adenoid hypertrophy rat model,while the blank group was replaced the equivalent amount of 0.9%sodium chloride solution.After molding,the montelukast group was gavaged with montelukast sodium particles,the TCM group was gavaged with Yunpi Huatan Tongqiao Decoction,model control group and blank group received equal amount of 0.9%sodium chloride solution,the course of treatment would all be 8 weeks.After molding and after the course of treatment,the symptom performance of model animals was assessed by animal behavioral score and the eosinophil percentage(EOS%),interleukin-4(IL-4),immunoglobulin E(IgE)and hypoxia-inducible factor-1α(HIF-1α)levels in blood serum and nasopharyngeal mucosal tissues were tested,and the pathomorphological changes of nasal and pharyngeal mucosa were observed by hematoxylin-eosin(HE)staining.[Results]After molding,the levels of animal behavioral score,EOS%,serum and tissue IL-4,IgE,and HIF-1α level in model control group,montelukast group and TCM group were significantly upregulated compared with blank group(P<0.01),and the nasal and pharyngeal mucosa showed different degrees of disease-related histopathological changes.After treatment,the levels of each index in montelukast group and TCM group were lower compared with that before treatment,and were also lower than that in model control group,all differences were statistically significant(P<0.05,P<0.01),and the histopathological damage was relieved than before the treatment.[Conclusion]The simulated rat model is similar to adenoid hypertrophy in terms of symptom manifestations and pathological changes,and effective drugs used clinically have similar efficacy in simulated rat models.In terms of predictive validity,the animal model of simulated adenoid hypertrophy can be made by combining animal model of allergic rhinitis and chronic pharyngitis,but it still needs further exploration and improvement.关键词
预测效度/腺样体肥大/过敏性鼻炎/慢性咽炎/动物模型/缺氧诱导因子/孟鲁司特钠/运脾化痰通窍方Key words
predictive validity/adenoid hypertrophy/allergic rhinitis/chronic pharyngitis/animal model/HIF-1α/Montelukast/Yunpi Huatan Tongqiao Decoction分类
医药卫生引用本文复制引用
张奕星,刘安琪,孙程辉,姜之炎..从预测效度角度探讨腺样体肥大动物模型的建立[J].浙江中医药大学学报,2025,49(2):132-140,9.基金项目
国家自然科学基金项目(82174433) （82174433）
浙江省中医药科技计划项目(2024ZL204) National Natural Science Foundation Project(82174433) （2024ZL204）
Zhejiang Province Traditional Chinese Medicine Science and Technology Project(2024ZL204) （2024ZL204）
