植物保护学报2025，Vol.52Issue(1)：52-59,8.DOI:10.13802/j.cnki.zwbhxb.2025.2024066
我国新发现夜蛾黑卵蜂孤雌产雌品系及其体内沃尔巴克氏体分子检测
First discovery of a thelytokous strain of egg parasitoid Telenomus remus in China and molecular detection of its endosymbiont Wolbachia
摘要
Abstract
To determine the Wolbachia infection status of a newly collected thelytokous strain of egg parasitoid Telenomus remus and its influence on reproductive mode,we conducted polymerase chain re-action(PCR)amplification using specific primers targeting the Wolbachia surface protein(wsp)gene and the cell division protein gene ftsz.Phylogenetic analysis was performed based on both single-gene and multilocus sequence typing method.Additionally,we investigated the effect of high-temperature on the sex ratio of T.remus.The results confirmed Wolbachia infection in the thelytokous strain of T.re-mus,with the wsp sequence exhibiting 100%similarity to that of strain wNaw from T.nawa.Phyloge-netic tree analysis consistently placed the Wolbachia strain infecting T.remus within supergroup A,in agreement with the ftsz gene-specific primerdetection results.Following high-temperature treatment,the proportion of male offspring increased progressively across generations,with male emergence rates of 9.23%in the F1 generation and 94.45%in the F2 generation.A small number of intersex individuals were also observed.This study represents the first report of a thelytokous strain of T.remus collected from the field in China.The discovery of this strain provides a novel natural enemy resource for the biological control of agricultural pests.关键词
夜蛾黑卵蜂/孤雌产雌品系/沃尔巴克氏体/分子检测Key words
Telenomus remus/thelytokous strain/Wolbachia/molecular detection引用本文复制引用
赵静,谭永安,谢永辉,宁德凯,王德海,陈华燕,石萍丽,肖留斌..我国新发现夜蛾黑卵蜂孤雌产雌品系及其体内沃尔巴克氏体分子检测[J].植物保护学报,2025,52(1):52-59,8.基金项目
云南省烟草公司科技计划(2023530000241011),江苏省自主创新(CX(22)5003),云南省大观实验室项目(YNDG202402YY03) （2023530000241011）
