中国中西医结合杂志2025，Vol.45Issue(3)：330-336,7.DOI:10.7661/j.cjim.20250114.064
青光安Ⅱ号方通过Bm-3b调控Bax/Bcl-2/Caspase-3抑制青光眼RGCs凋亡的机制研究
Mechanism of Qingguangan Ⅱ Formula Inhibiting RGCs Apoptosis in Glaucoma via Brn-3b/Bax/Bcl-2/Caspase-3 Regulation
摘要
Abstract
Objective To explore the mechanism by which Qingguangan Ⅱ Formula(QGA Ⅱ)inhibits the apoptosis of retinal ganglion cells(RGCs)in glaucoma through the regulation of B-cell lymphoma 2(Bcl-2)/Bcl-2-associated X protein(Bax)/Cysteine-aspartic protease 3(Caspase-3)by Brain-specific homeobox/POU domain protein 3B(Brn-3b).Methods After one week of acclimatization sixty healthy male SPF SD rats were randomly divided into control group,model group,low-dose,medium-dose,and high-dose QGA Ⅱgroup,and Yimaikang group,10 in each group.Except for the control group,elevated intraocular pressure was induced in the other groups by cauterizing the episcleral veins.The control and model groups were treated with saline,while the Yimaikang(9.67 g·kg-1·d-1)and QGA Ⅱ(3.375,6.75,13.5 g·kg-1·d-1)groups received their respective treatments via gavage for 4 weeks.Intraocular pressure was measured preoperatively and at 30 minutes,1,7,14,and 28 days postoperatively.After euthanizing the rats,retinal tissues were isolated for histological examination and analysis of RGC apoptosis and the expression of Brn-3b,Caspase-3,Bcl-2,and Bax proteins and mRNA.Results Intraocular pressure was significantly elevated in all model groups compared to baseline(P＜0.05)and remained high at 4 weeks.Compared to the model group,the QGA Ⅱ and Yimaikang groups exhibited significantly reduced RGC apoptosis(P＜0.01).Among them,the high-dose QGAⅡ group showed the lowest RGC apoptosis rates(P＜0.05).Compared to the model group,expressions of Caspase-3 and Bax proteins and mRNA increased in the Yimaikang and high-dose QGA Ⅱ groups(P＜0.01),while expressions of Brn-3b and Bcl-2 proteins and mRNA significantly decreased(P＜0.01).Conclusion QGA Ⅱinhibits RGC apoptosis by regulating Brn-3b,Bax/Bcl-2/Caspase-3,with efficacy comparable to Yimaikang.关键词
青光安Ⅱ号方/脑特定蛋白3B/B淋巴细胞瘤-2基因/Bcl-2相关X蛋白/半胱氨酸天冬氨酸蛋白酶-3/视网膜神经节细胞/凋亡Key words
Qingguangan Ⅱ formula/Brn-3b/Bax/Bcl-2/Caspase-3/RGCs/apoptosis引用本文复制引用
李文娟,谭涵宇,时健,彭榆津,周蓓,彭清华..青光安Ⅱ号方通过Bm-3b调控Bax/Bcl-2/Caspase-3抑制青光眼RGCs凋亡的机制研究[J].中国中西医结合杂志,2025,45(3):330-336,7.基金项目
国家自然科学基金面上基金(No.82274588) （No.82274588）
湖南省中医药科研课题(No.B2023093) （No.B2023093）
