中国中西医结合杂志2025，Vol.45Issue(3)：337-342,6.DOI:10.7661/j.cjim.20240914.214
荷叶提取物抑制粪肠球菌浮游细菌及其生物膜
Lotus Leaves Extracts Inhibits Planktonic Enterococcus faecalis and Its Biofilm
摘要
Abstract
Objective To investigate the effects of lotus leaf extracts on the planktonic bacteria and biofilm of Enterococcus faecalis.Methods Lotus leaf water extract,ethanol extract,total flavonoid extract,and crude alkaloid extract were prepared.The minimum inhibitory concentration(MIC)of each lotus leaf extract against Enterococcus faecalis was determined by two-fold gradient dilution and plate coating methods.Based on the results,the experimental groups were divided into 1.3 g/mL and 0.65 g/mL crude alkaloid extract groups,positive control group(2％chlorhexidine solution),and negative control group(sterile PBS solution).The LIVE/DEAD BacLightTM Bacterial Viability Kit was used for fluorescence staining of live/dead bacteria,and the formation of Enterococcus faecalis biofilm after treatment with the crude alkaloid extract was observed using confocal laser scanning microscopy(CLSM).The structure of live and dead bacteria in the biofilm was observed under a fluorescence microscope after acridine orange/ethidium bromide staining.Results The MICs of lotus leaf water extract,ethanol extract,total flavonoid extract,and crude alkaloid extract against Enterococcus faecalis were 7.04,1.76,＞1.3,and 1.3 g/mL,respectively.In the formation and structure of Enterococcus faecalis biofilm,compared with the negative control group,the percentage of live bacteria in the 1.3 g/mL and 0.65 g/mL crude alkaloid extract groups and the positive control group significantly reduced(P＜0.01).Compared with the positive control group,the percentage of live bacteria in the 0.65 g/mL crude alkaloid extract group increased(P＜0.01),while there was no statistically significant difference in the 1.3 g/mL crude alkaloid extract group(P＞0.05).Conclusion The crude alkaloid extract of lotus leaves can effectively inhibit the growth of Enterococcus faecalis and the formation and structure of its biofilm,suggesting good potential for application in root canal therapy.关键词
荷叶提取物/粪肠球菌/生物膜/死/活菌染色/中药提取物Key words
lotus leaf extract/Enterococcus faecalis/biofilm/live/dead bacterial staining/Chinese herbal extracts引用本文复制引用
潘利君,胡翔宇,李宗莉,赵瑜,邓黎超,潘乙怀..荷叶提取物抑制粪肠球菌浮游细菌及其生物膜[J].中国中西医结合杂志,2025,45(3):337-342,6.基金项目
温州市重大科技创新攻关项目(No.ZY2022019) （No.ZY2022019）
浙江省基础公益研究计划项目(No.LGF21H140005) （No.LGF21H140005）
