中药材2024，Vol.47Issue(4)：851-856,6.DOI:10.13863/j.issn1001-4454.2024.04.009
不同生长年限唐古特大黄根转录组学分析及蒽醌类生物合成基因挖掘
Transcriptomic Analysis and Anthraquinone Biosynthesis Gene Mining of Rheum tanguticum with Different Growth Years
摘要
Abstract
Objective:To analyze the transcriptome changes of Rheum tanguticum in different growth years and identify the key genes involved in anthraquinone biosynthesis,so as to elucidate the molecular regulation mechanism of anthraquinone biosynthesis.Methods:High-throughput sequencing technology,gene function annotation,homology search and qRT-PCR verification were used to identify the genes involved in the biosynthesis of anthraquinone compounds of Rheum tanguticum.Results:97 499 unigenes were assem-bled from the original data with an N50 of 2 371 bp,of which 69.42%unigenes were annotated.A total of 7 349 differentially expressed genes were screened in the samples of different growth years,and the number of differentially expressed genes was less between 5-year olds and 8-year olds.251 unigenes involved in anthraquinone biosynthesis,25 of which were differentially expressed in at least one con-trol group.The expression of some genes was verified by qRT-PCR,which was consistent with the results of transcriptome analysis.Eight PKS Ⅲ genes were further screened,four of which were CHS-like genes.PKS Ⅲ 6 and PKS Ⅲ 7 showed high expressions at the peak of anthraquinone accumulation.Conclusion:It is preliminarily speculated that PKS Ⅲ 6 and PKS Ⅲ 7 may be involved in the accumula-tion of anthraquinone in roots,and this result will be helpful for the subsequent research on the synthesis mechanism of anthraquinone components in Rheum tanguticum.关键词
唐古特大黄/转录组/不同生长年限/蒽醌生物合成/差异表达基因Key words
Rheum tanguticum Maxim.ex Balf./Transcriptome/Different growth years/Anthraquinone biosynthesis/Differentially expressed gene分类
中医学引用本文复制引用
赵硕,王玲玲,李嘉楠,陈晨,周国英..不同生长年限唐古特大黄根转录组学分析及蒽醌类生物合成基因挖掘[J].中药材,2024,47(4):851-856,6.基金项目
中国科学院-青海省国家公园联合专项(LHZX-2020-09) （LHZX-2020-09）
青海省科技厅成果转化项目(2021-SF-149) （2021-SF-149）
