中药材2024，Vol.47Issue(4)：922-926,5.DOI:10.13863/j.issn1001-4454.2024.04.021
合苞橐吾指纹图谱的建立及含量测定
Fingerprint Establishment and Content Determination of Ligularia schmidtii Leaves
摘要
Abstract
Objective:To establish HPLC fingerprint of Ligularia schmidtii leaves and to determine the contents of 4 components.Methods:HPLC fingerprint of 10 batches of Ligularia schmidtii leaves was established by"Traditional Chinese medicine chromatograph-ic fingerprint similarity evaluation system(2004 A edition)",and similarity evaluation was carried out.SPSS 23.0 and SIMCA 14.1 software were used for cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis.With the variable importance projection(VIP)value greater than 1 as the standard,the markers affecting the quality of Ligularia schmidtii leaves were screened,the content of the compounds were determined.Results:There were 20 common peaks in the fingerprints of 10 batches of Ligularia schmidtii leaves,and the similarity was 0.845 to 0.999.Four components were identified,namely chlorogenic acid,rutin,lute-olin and isochlorogenic acid A,the contents of which were 17.42～36.62 mg/g,0.99～1.81 mg/g,0.36～0.67 mg/g and 1.03～2.17 mg/g,respectively.Conclusion:The established HPLC fingerprint and component content determination method of Ligularia schmidtii leaves is simple and accurate,and can be used for the quality evaluation of Ligularia schmidtii.关键词
合苞橐吾/指纹图谱/含量测定/HPLC法/化学模式识别分析Key words
Ligularia schmidtii(Maxim.)Makino/Fingerprint/Content determination/HPLC/Chemical pattern recognition analy-sis分类
医药卫生引用本文复制引用
赵娣,马超,李琪,姜丽,张春芳,吕惠子..合苞橐吾指纹图谱的建立及含量测定[J].中药材,2024,47(4):922-926,5.基金项目
吉林省地方药材标准项目(JLYC-2020-016) （JLYC-2020-016）
