中药材2024，Vol.47Issue(4)：963-968,6.DOI:10.13863/j.issn1001-4454.2024.04.028
松油烯-4-醇调节棕榈酸诱导胰岛β细胞内质网应激抑制细胞凋亡
Study on the Effect of Terpine-4-ol on the Regulation of Endoplasmic Reticulum Stress Induced by Palmitic Acid to Inhibit Apoptosis of Pancreatic β Cells
摘要
Abstract
Objective:To study the effect and mechanism of terpinen-4-ol on regulation of endoplasmic reticulum stress induced by palmitic acid(PA)and inhibition of cell apoptosis in Min6 cells.Methods:For analyzing the effects of terpine-4-ol on endoplasmic re-ticulum stress and apoptosis of Min6 cells induced by palmitic acid,the experiments were divided into normal control group,model group,metformin(100 μmol/L)group,low(5 μmol/L)and high(20 μmol/L)concentration of terpine-4-ol groups.For analyzing the mechanism,the experiment was divided into normal control group,model group,endoplasmic reticulum stress inhibitor 4-phenylbutyric acid(4-PBA,25 mmol/L)group,terpine-4-ol(20 μmol/L)group,4-PBA(25 mmol/L)with terpine-4-ol(20 μmol/L)group.Cell via-bility was detected by MTT.Cytoplasmic calcium content and apoptosis rate were detected by flow cytometry.The expression levels of endoplasmic reticulum stress markers p-PERK,ATF4,GRP78 and apoptosis markers CHOP,Caspase-3,Caspase-9 and Bax were detec-ted by Western Blot.Results:Compared with normal control group,cytoplasmic calcium ions and apoptosis rate were significantly in-creased,cell activity was significantly decreased,and protein expressions of CHOP,Caspase-3,Caspase-9,Bax,ATF4,GRP78 and the level of p-PERK/PERK were significantly increased in model group(P<0.01).Compared with model group,cytoplasmic calcium ion and apoptosis rate were significantly decreased,cell activity was significantly increased,protein expressions of CHOP,Caspase-3,Caspase-9,Bax,ATF4,GRP78 and the level of p-PERK/PERK were significantly decreased(P<0.05 or P<0.01).There was no signif-icant difference between 4-PBA with terpine-4-ol group and 4-PBA group(P>0.05).Conclusion:Terpine-4-ol can inhibit palmitic acid-induced apoptosis of Min6 cells by regulating endoplasmic reticulum stress.关键词
松油烯-4-醇/棕榈酸/内质网应激/细胞凋亡/胰岛β细胞Key words
Terpine-4-ol/Palmitic acid/Endoplasmic reticulum stress/Cell apoptosis/Pancreatic β cells分类
医药卫生引用本文复制引用
陈虹雨,李金锦,尚雪祎,张闯,张盈,沈祥春,张彦燕..松油烯-4-醇调节棕榈酸诱导胰岛β细胞内质网应激抑制细胞凋亡[J].中药材,2024,47(4):963-968,6.基金项目
国家自然科学基金资助项目(82060775) （82060775）
贵州医科大学高层次人才启动基金项目(校博合J字[2021]010号) （校博合J字[2021]010号）
贵州省科技创新基地(黔科合中引地[2023]003) （黔科合中引地[2023]003）
