中药材2024，Vol.47Issue(4)：969-976,8.DOI:10.13863/j.issn1001-4454.2024.04.029
茱萸丸矫正"免疫-炎症"网络失衡改善动脉粥样硬化的机制研究
Study on Mechanism of Zhuyu Pills to Improve Atherosclerosis by Correcting"Immune-Inflammation"Imbalance
摘要
Abstract
Objective:To investigate the effect of Zhuyu pills on atherosclerotic plaque and its possible mechanism.Methods:The ApoE-/-mice atherosclerosis model was establish by fed high fat diet.Fifty ApoE-/-mice were divided into model group,atorvastatin calcium(10.40 mg/kg)group and Zhuyu pills low(130.54 mg/kg),medium(261.08 mg/kg)and high(522.16 mg/kg)dose groups with 10 mice in each group according to random number table method.Ten C57BL/6J mice were taken as normal control group and fed with ordinary diet.Each administration group was given the corresponding drugs by intragastric administration,and the mice in normal control group and model group were given the corresponding volume of normal saline by intragastric administration,once a day,continu-ous intragastric administration for 12 weeks.The levels of TC,TG,LDL-C and HDL-C in liver tissue were detected by biochemical meth-od.The fasting blood glucose level was detected by automatic biochemical analyzer.The histopathological changes of aorta,liver and adi-pose tissue were observed by HE staining.Serum levels of insulin,TNF-α,IL-6,IL-4 and IL-10 were detected by ELISA.The expression of CD1d protein in liver tissue was detected by immunofluorescence.The changes of iNKT cells in liver were detected by flow cytome-try.The expressions of CD1d mRNA in liver and TNF-α,IL-6,IL-4 and IL-10 mRNA in aorta tissue were detected by real-time PCR.Results:Compared with the normal control group,the levels of TC,TG and LDL-C in liver tissue of model group were significantly in-creased,HDL-C level was significantly decreased,insulin resistance index was significantly increased(P<0.01),aortic plaque area was obviously increased,lipopathia and large amount of fat droplets appeared in liver,fat cell volume expanded,and serum levels of TNF-αand IL-6 were significantly increased.The levels of IL-4 and IL-10 were significantly decreased,the expressions of CD1d mRNA and protein in liver tissue were significantly increased,the number of iNKT was significantly increased,the mRNA expressions of TNF-α and IL-6 in aortic tissue were significantly increased,and the mRNA expressions of IL-4 and IL-10 were significantly decreased(P<0.01).Compared with model group,the levels of TC,TG and LDL-C in liver tissue were significantly decreased,HDL-C level was significantly increased,insulin resistance index was significantly decreased(P<0.05 or P<0.01),aortic plaque area was obviously reduced,liver lip-id deposition was obviously reduced,lipid droplets were obviously reduced,and adipocyte volume was obviously reduced.Serum TNF-α and IL-6 levels were significantly decreased,IL-4 and IL-10 levels were significantly increased,CD1d mRNA and protein expressions in liver tissue were significantly decreased,iNKT number was significantly decreased,and TNF-α and IL-6 mRNA expressions in aortic tis-sue were significantly decreased.The mRNA expressions of IL-4 and IL-10 were significantly increased(P<0.01).Conclusion:Zhuyu pills have a good therapeutic effect on atherosclerotic plaque in mice,and its mechanism may be related to regulating CD1d-iNKT cell axis and reducing inflammatory infiltration.关键词
动脉粥样硬化/茱萸丸/CD1d-iNKT细胞轴/苦辛化浊Key words
Atherosclerosis/Zhuyu pills/CD1d-iNKT cell axis/Combination of bitter and occinative drugs to reduce fat turbidity分类
医药卫生引用本文复制引用
宋玮,沈涛..茱萸丸矫正"免疫-炎症"网络失衡改善动脉粥样硬化的机制研究[J].中药材,2024,47(4):969-976,8.基金项目
国家自然科学基金项目(81973743) （81973743）
四川省科技厅重点研发项目(22ZYF0905) （22ZYF0905）
