遵义医科大学学报2025，Vol.48Issue(3)：219-226,233,9.
慢性束缚应激致抑郁小鼠模型中内侧前额叶皮层与小脑髓鞘化的年龄差异化研究
Age-dependent differences of medial prefrontal cortex and cerebellum myelina-tion in the chronic restraint stress-induced depression mouse model
摘要
Abstract
Objective To investigate the age-dependent differences of central nervous system(CNS)myelina-tion in the chronic restraint stress(CRS)-induced depression mouse model.Methods Male C57 mice of 3 month(adult)and postnatal 7(P7,juvenile)age were performed CRS for 21 days to generate the depression model.Behavior tests were performed to monitor the depression-like behaviors in the CRS mouse models at different a-ges.Immunohistochemistry(IHC)was used to examine the myelin structure changes in the medial prefrontal cortex(mPFC)and cerebellum(CB)of CRS mice at different ages.Western blotting(WB)was used to moni-tor the expression of myelin-related proteins in the mPFC and CB.Furthermore,WB and IHC were performed to reveal the possible mechanism under depression-related myelination dysfunction.Results Behavior tests showed that both adult and juvenile depression mice exhibited depression-like behaviors,meanwhile,the juvenile models showed hyperactivity.MBP staining showed decreased myelin structure in the mPFC of both adult and juvenile CRS mice,while the enhanced myelin structure was only observed in the CB of the juvenile CRS mice.WB re-vealed that CRS caused decreased expression level of myelin-associated proteins MBP and MOG in the mPFC of both adult and juvenile CRS mice,but increased MBP and MOG level in the CB of the juvenile CRS mice.Addi-tionally,WB showed that the expression of Lingo-1 and MCT1,which are the key molecules to mediate to crosstalk between neuron and myelin,were disrupted in the mPFC of both adult and juvenile CRS mice,along with the decreased neural activity revealed by c-Fos staining.Conclusion Myelination defect exhibited in the mPFC of both adult and juvenile CRS mice,which may be attributed to the blunted neural activity caused by dis-rupted interaction between neuron and myelin in the CRS mPFC.关键词
慢性束缚应激/抑郁/髓鞘化/年龄差异/内侧前额叶皮层Key words
chronic restraint stress/depression/myelination/age-dependent differences/medial prefrontal cortex分类
基础医学引用本文复制引用
钟富旺,易如岚,周亮..慢性束缚应激致抑郁小鼠模型中内侧前额叶皮层与小脑髓鞘化的年龄差异化研究[J].遵义医科大学学报,2025,48(3):219-226,233,9.基金项目
国家自然科学基金资助项目(NO:82460242). （NO:82460242）
