遵义医科大学学报2025，Vol.48Issue(3)：234-242,9.
Bscl2基因敲除对细胞增殖和DNA损伤修复能力的影响
Effects of Bscl2 gene knockout on cell proliferation and DNA damage repair capacity
摘要
Abstract
Objective This study aims to investigate whether the knockout of the Bscl2 gene affects cell prolifer-ation and DNA damage repair capabilities or not both in vivo and in vitro.Methods Construction of a Bscl2 gene knockout mouse model and MEFs was isolated from fetuses at embryonic day 14.5(E14.5 d)by using trypsin digestion.The isolated and cultured MEFs were purified and identified through immunofluorescence staining.The genotypes of the cultured cells and mice was identified by combining PCR amplification with agarose gel e-lectrophoresis,and cells and mice were categorized into the wild type(WT)and Bscl2-knockout(KO)groups.Trypan blue staining and 5-bromo-2′-deoxyuridine(BrdU)immunofluorescence labeling was performed to evalu-ate cell proliferation.Assessment of DNA damage repair capacity in two groups of cells and mouse tissues after double-strand break induction was performed by γH2AX immunofluorescence staining.Results MEFs from E14.5 d was successfully isolated to establish a mouse MEF cell line.The proliferation capacity of Bscl2-knock-out MEFs was significantly lower than that of the wild type.DNA damage repair capacity in Bscl2-knockout MEFs and mouse tissues was markedly impaired when comparing to the wild type.Conclusion Knockout of the Bscl2 gene inhibits the proliferation of mouse MEFs and reduces the DNA damage repair capacity of mouse cells and tissues.关键词
先天性脂肪营养不良2型基因/胚胎成纤维细胞/细胞增殖/DNA双链断裂/DNA修复Key words
berardinelli-seip congenital lipodystrophy type 2/embryonic fibroblasts/cell proliferation/DNA double-strand breaks/DNA repair分类
临床医学引用本文复制引用
张丛俏,黄翔,张伟杨,安智渊,田盼,万能,任来峰,郭晋锋..Bscl2基因敲除对细胞增殖和DNA损伤修复能力的影响[J].遵义医科大学学报,2025,48(3):234-242,9.基金项目
山西省自然科学基金资助项目(NO:202403021211128) （NO:202403021211128）
山西省卫生健康委科研基金(NO:2024108). （NO:2024108）
