中药与临床2025，Vol.16Issue(2)：9-16,8.
基于UPLC-Q-TOF-MS结合非靶向代谢组学技术评价枳壳不同饮片的成分变化
Evaluation of compositional changes in differently processed decoction pieces of Aurantii Fructus(Zhiqiao)based on UPLC-Q-TOF-MS coupled with non-targeted metabolomics techniques
摘要
Abstract
Objective:To investigate the effects of traditional decoction pieces,fresh-cut preparation and different processing methods on chemical components of Zhiqiao.Methods:The components of Zhiqiao decoction pieces were determined by non-targeted metabolomics technique combined with UPLC-Q-TOF-MS.PCA analysis and OPLS-DA analysis were used to analyze three kinds of Zhiqiao decoction pieces and find out the different components.Results:A total of 69 compounds,including 17 flavonoids,6 alkaloids,5 coumarins,17 organic acids,1 amino acid,1 quinone,and 22 other substances,were detected in Zhiqiao decoction pieces.Seven differential components including berberine,7-hydroxycoumarin,citral,naringeninchalcone,hesperidin,naringin and poncirin,were identified,among which the relative contents of berberine,7-hydroxycoumarin,and citral appeared to have a significant decrease,whereas the flavonoid components,such as hesperidin and naringenin,increased significantly.Conclusion:The chemical constituents in Zhiqiao show significant changes during the processing of the decoction pieces,among which the flavonoid constituents rise significantly,which help Zhiqiao to better exert its therapeutic effects.关键词
枳壳/黄酮/非靶向代谢组学/主成分分析Key words
Zhiqiao/flavonoids/non-targeted metabolomics/principal component analysis分类
医药卫生引用本文复制引用
付琴文,杨鹭,王瑾,黄勤挽..基于UPLC-Q-TOF-MS结合非靶向代谢组学技术评价枳壳不同饮片的成分变化[J].中药与临床,2025,16(2):9-16,8.基金项目
四川省中医药管理局课题(2023MS355,2023MS400) （2023MS355,2023MS400）
