中药与临床2025，Vol.16Issue(2)：28-33,6.
地奥心血康对阿霉素诱导的斑马鱼心脏毒性保护作用的研究
Protective effect of Di'ao Xinxuekang on doxorubicin-induced cardiotoxicity in zebrafish
摘要
Abstract
Objective:To investigate the protective effect of Di'ao Xinxuekang(DXXK)against doxorubicin-induced cardiotoxicity in zebrafish and its effect on the expression of apoptosis-related genes.Methods:48 hpf transgenic zebrafish(cmlc2∶EGFP)were randomly divided into the control group and the group with different concentrations of DOX and DXXK,to observe the survival and heart rate of the zebrafish after 48 hours of treatment.After co-treatment of zebrafish with DOX(60 μM)and DXXK(1,2 and 4 μg·mL-1)for 48 hours,the survival rate and pericardial oedema of zebrafish were observed under the white light condition of in the fluorescence microscope,and the pericardial area and SV-BA(venous sinus and arterial ball)distance were calculated.The diastolic and systolic processes of the heart were recorded under fluorescence microscopy,and the heart rate and stroke volume(SV)of zebrafish embryos were calculated.RT-qPCR was used to detect the mRNA expressions of Bax,Bcl-2 and Caspase-3.Results:Compared with the control group,DOX treatment decreased survival,reduced mobility,caused pericardial oedema,and increased pericardial area,decreased atrial-ventricular overlap area,and increased SV-BA distance.And DOX decreased heart rate and stroke volume in zebrafish(P<0.001 or P<0.01).In contrast,co-treatment of DXXK with DOX improved the rate of survival,alleviated pericardial oedema,reduced pericardial area,shortened the SV-BA distance,and significantly increased heart rate and SV(P<0.001 or P<0.01).RT-qPCR results showed that DOX significantly increased the mRNA expressions of the pro-apoptotic Bax and Caspase 3,and decreased the mRNA expression of the anti-apoptotic Bcl-2,while the expressions of the above apoptotic genes was reversed by DXXK treatment.Conclusion:DXXK may protect against DOX-induced cardiotoxicity in zebrafish,and this effect may be related to affecting the mRNA expression of apoptosis.关键词
地奥心血康/阿霉素/心脏毒性/斑马鱼Key words
Di'ao xinxuekang/doxorubicin/cardiotoxicity/zebrafish分类
医药卫生引用本文复制引用
邓良艳,刘佳,瞿礼萍,邹文俊..地奥心血康对阿霉素诱导的斑马鱼心脏毒性保护作用的研究[J].中药与临床,2025,16(2):28-33,6.基金项目
国家自然科学基金(82274324) （82274324）
四川省自然科学基金杰出青年科学基金(24NSFJQ0125) （24NSFJQ0125）
