动物营养学报2025，Vol.37Issue(3)：1715-1726,12.DOI:10.12418/CJAN2025.145
饲粮中添加有机锰、锌和纳米硒-褐藻寡糖对鸡蛋蛋壳暗斑发生的影响
Effects of Dietary Supplementation of Organic Manganese,Zinc and Nano-Selenium Alginate Oligosaccharides on Occurrence of Eggshell Dark Spots in Eggs
摘要
Abstract
The aim of this study was to explore the effects of dietary supplementation with organic manganese,zinc,nano-selenium alginate oligosaccharides on the occurrence of egg shell dark spots in laying hens with a high incidence of such spots.A total of 150 Roman pink laying hens at 288 days of age were selected,among which 30 had a low incidence of dark spots and 120 had a high incidence.They were divided into the NC group(low dark spot+basal diet),the PC group(high dark spot+basal diet),the MnG group(high dark spot+basal diet+60 mg/kg manganese hydroxymethionine+60 mg/kg MnSO4),the ZnG group(high dark spot+basal di-et+55 mg/kg zinc hydroxymethionine+55 mg/kg ZnSO4),and the SeG group(high dark spot+basal diet+0.3 mg/kg nano-selenium alginate oligosaccharides),with 10 replicates per group and 3 chickens per replicate.The experiment lasted for 12 weeks.The results showed as follows:1)compared with the NC group,the egg-shell strength,eggshell moisture content,and mastoid layer thickness in the PC group were significantly in-creased(P＜0.05),while the eggshell calcium content,the crude protein content of the eggshell membrane,and the total amino acid content were significantly decreased(P＜0.05).The relative mRNA expression of egg-shell gland calcium-binding protein-D28K(CaBP-D28k)was significantly reduced(P＜0.05).2)Compared with the PC group,the egg dark spot score mean in all the supplemented groups was significantly decreased(P＜0.05).3)Compared with the PC group,the thickness of the eggshell mastoid layer in the MnG and SeG groups was significantly decreased(P＜0.05),and the content of crude protein in the eggshell membrane was significantly increased(P＜0.05).Additionally,the contents of essential amino acids and total amino acids of the eggshell membrane in the MnG and ZnG groups were significantly increased(P＜0.05).4)Compared with the PC group,the relative mRNA expression levels of carbonic anhydrase(CA)and CaBP-D28k in the egg-shell gland in the MnG group were significantly up-regulated(P＜0.05),and the relative mRNA expression levels of estrogen receptor α(ERα)in the eggshell gland in the ZnG and SeG groups were significantly up-regulated(P＜0.05).In conclusion,feeding laying hens with a high incidence of dark spots with diets supple-mented with 120 mg/kg manganese(60 mg/kg manganese hydroxymethionine+60 mg/kg MnSO4),110 mg/kg zinc(55 mg/kg zinc hydroxymethionine+55 mg/kg ZnSO4),or 0.3 mg/kg nano-selenium algi-nate oligosaccharides could improve the eggshell structure,reduce water accumulation in the eggshell by in-creasing the eggshell calcium content and the crude protein content of the eggshell membrane,and enhance the function of the eggshell gland by increasing the expression of genes related to calcium metabolism in the egg-shell gland,thereby reducing the occurrence of eggshell dark spots.[Chinese Journal of Animal Nutrition,2025,37(3):1715-1726]关键词
暗斑/蛋品质/蛋壳质量/蛋壳腺功能/蛋鸡Key words
dark spots/egg quality/eggshell quality/uterine function/laying hens分类
农业科技引用本文复制引用
蔡春燕,张克英,曾秋凤,白世平,丁雪梅,彭焕伟,刘艳,王建萍..饲粮中添加有机锰、锌和纳米硒-褐藻寡糖对鸡蛋蛋壳暗斑发生的影响[J].动物营养学报,2025,37(3):1715-1726,12.基金项目
国家重点研发计划(2021YFD300204) （2021YFD300204）
四川省科技计划项目(2022YFH0070) （2022YFH0070）
