动物营养学报2025，Vol.37Issue(3)：1771-1783,13.DOI:10.12418/CJAN2025.150
白头翁口服液对脂多糖攻毒无特定病原体白来航鸡肠道炎症以及氧化应激的影响
Effects of Baitouweng Oral Liquid on Intestinal Inflammation and Oxidative Stress of Lipopolysaccharide Challenge Specific Pathogen Free White Leghorn Chickens
摘要
Abstract
The aim of this study was to construct oxidative stress model animals by attacking specific pathogen free(SPF)white Leghorn chickens,and then evaluate the effects of Baitouweng oral liquid(BTW)on the immune and antioxidant capacity of broilers in poultry production.Thirty 21-day-old SPF male white Leghorn chickens were randomly assigned to 3 groups:control group(CON group),lipopolysaccharide(LPS)group(LPS group)and BTW+LPS group(B-L group)with 10 replicates per group and 1 chicken per replicate.In CON group,2 mL normal saline was injected intraperitoneally at 31,33 and 35 days of age.The LPS groups were intraperitoneally injected with LPS(0.5 mg/kg of BW)at 31,33 and 35 days of age.Chickens in the B-L group were given 4 mL BTW per day at 21 to 35 days of age,and LPS(0.5 mg/kg of BW)was intraper-itoneally injected at 31,33 and 35 days of age.The experiment lasted for 14 days.The results showed that compared with CON group,LPS group showed obvious diarrhea symptoms,serum reactive oxygen species(ROS)and malondialdehyde(MDA)contents were significantly increased(P＜0.05),and total antioxidant capacity(T-AOC)was significantly decreased(P＜0.05),indicating that the oxidative stress model of SPF chickens induced by LPS was successfully established.Immune index results showed that compared with LPS group,BTW intake significantly increased blood hemoglobin(HGB)level and red blood cell count(RBC)of model chickens(P＜0.05).The contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in serum of model chickens were significantly decreased(P＜0.05).The contents of inter-leukin-4(IL-4)and interleukin-10(IL-10)in serum were significantly increased(P＜0.05),and were main-tained at similar levels to those in CON group(P＞0.05).The results of antioxidant indexes showed that com-pared with LPS group,the activities of glutathione peroxidase(GSH-Px),catalase(CAT)and superoxide dismutase(SOD)in serum of model chickens were significantly increased by BTW intake(P＜0.05).At the same time,BTW also maintained the normal morphology of ileum mucosa,liver and immune organ index of model chickens.In summary,this study confirms that BTW can improve the immune and antioxidant capacity of model chickens by regulating the contents of inflammatory cytokines and the activities of antioxidant en-zymes in poultry,as well as affecting the development of tissues and organs,so as to alleviate intestinal inflam-mation and oxidative stress of model chickens.[Chinese Journal of Animal Nutrition,2025,37(3):1771-1783]关键词
SPF白来航鸡/氧化应激/免疫/抗氧化/白头翁口服液Key words
SPF white Leghorn chicken/oxidative stress/immunity/antioxidant/Baitouweng oral liquid分类
农业科技引用本文复制引用
张朝生,陈志敏,王少龙,王琰,刘国华..白头翁口服液对脂多糖攻毒无特定病原体白来航鸡肠道炎症以及氧化应激的影响[J].动物营养学报,2025,37(3):1771-1783,13.基金项目
科技创新2023-重大项目(2023ZD0406307) （2023ZD0406307）
