动物营养学报2025，Vol.37Issue(3)：1929-1939,11.DOI:10.12418/CJAN2025.163
饲料中添加色氨酸和蛋氨酸对中华绒螯蟹生长性能、消化能力、免疫功能及抗氧化能力的影响
Effects of Dietary Tryptophan and Methionine on Growth Performance,Digestive Ability,Immune Function and Antioxidant Capacity of Chinese Mitten Crab(Eriocheir sinensis)
摘要
Abstract
This experiment was conducted to investigate the effects of dietary tryptophan and methionine on growth performance,digestive ability,immune function and antioxidant capacity of Chinese mitten crab(Erio-cheir sinensis).In a two-factor experimental design,0,0.31%and 0.63%L-Try and 0,0.33%and 0.67%L-Met were added to the basal diet,nine groups of isonitrogen and isolipid diets were prepared with three Try lev-els(actual contents were 0.31%,0.62%and 0.94%)and three Met levels(actual contents were 0.66%,0.99%and 1.33%),and each group contained 3 replicates.The Eriocheir sinensis with initial body weight of(50.27±2.20)g were selected,and the stocking density was 4 500 crab/hm2.The pre-experimental period lasted for 10 days,and the experimental period lasted for 80 days.The results showed as follows:1)dietary Try and Met levels had not significant interaction effect on the growth performance of Eriocheir sinensis(P＞0.05).The weight gain rate,specific growth rate and protein efficiency of 0.94%Try level and 0.99%Met level were significantly higher than those of other levels(P＜0.05),and the feed conversion rate was signifi-cantly lower than that of other levels(P＜0.05).2)Dietary Try and Met levels had significant interaction effects on muscle crude protein and rude ash content of Eriocheir sinensis(P＜0.05).The muscle crude protein content of 0.94%Try level and 0.99%and 1.33%Met level was significantly higher than that of other levels(P＜0.05),and the muscle crude ash content was significantly lower than that of other levels(P＜0.05).3)Dietary Try and Met levels had significant interaction effects on hepatopancreas pepsin,trypsin,lipase and am-ylase activities of Eriocheir sinensis(P＜0.05).The activities of pepsin and trypsin in hepatopancreas of 0.94%Try level and 0.99%Met level were significantly higher than those of other levels(P＜0.05),the activities of lipase and amylase in hepatopancreas of 0.62%Try level and 0.99%Met level were significantly higher than those of other levels(P＜0.05).4)Dietary Try and Met levels had significant interaction effects on the serum non-specific immune(except acid phosphatase)and antioxidant indexes of Eriocheir sinensis(P＜0.05).In the non-specific immune indexes,the activities of alkaline phosphatase and lysozyme in serum of 0.94%Try level and 0.99%Met level were significantly higher than those of other levels(P＜0.05).In the antioxidant in-dexes,the activities of catalase and superoxide dismutase in serum of 0.94%Try level were significantly higher than those of other levels(P＜0.05),and the malondialdehyde content was significantly lower than that of oth-er levels(P＜0.05);the activities of catalase,superoxide dismutase and total antioxidant capacity in serum of 0.99%Met level were significantly higher than those of other levels(P＜0.05).In conclusion,the appropriate dietary Try and Met can improve the growth performance of Eriocheir sinensis,and the Try and Met supple-mental levels and their interaction affect the muscle crude protein content,hepatopancreas digestive enzyme ac-tivity,non-specific immunity and antioxidant capacity;comprehensive all indexes,the best results are obtained when the levels of Try and Met in the diet are 0.94%and 0.99%,respectively.[Chinese Journal of Animal Nutrition,2025,37(3):1929-1939]关键词
中华绒螯蟹/色氨酸/蛋氨酸/生长/体成分/消化酶/免疫功能/抗氧化能力Key words
Eriocheir sinensis/tryptophan/methionine/growth/body composition/digestive enzymes/im-mune function/antioxidant capacity分类
农业科技引用本文复制引用
蒋湘辉,王兴兵,李赫,胡宗云,张健,魏洪祥,杨培民..饲料中添加色氨酸和蛋氨酸对中华绒螯蟹生长性能、消化能力、免疫功能及抗氧化能力的影响[J].动物营养学报,2025,37(3):1929-1939,11.基金项目
辽宁省科技厅"辽宁淡水虾蟹绿色养殖关键技术协同创新"(2022JH2/101300286) （2022JH2/101300286）
沈阳市农村科技特派团(23-411-2-17) （23-411-2-17）
