动物营养学报2025，Vol.37Issue(3)：2003-2018,16.DOI:10.12418/CJAN2025.170
羧甲基茯苓多糖、丁酸梭菌和罗伊氏乳杆菌后生元复合物对昆明小鼠生长性能和盲肠菌群结构的影响
Impacts of a Compound of Carboxymethylpachymaran and Clostridium butyricum and Lactobacillus reuteri Postbiotics on Growth Performance and Cecal Microflora Composition of Kunming Mice
摘要
Abstract
The objective of this research was to examine the impacts of a compound comprising carboxymeth-ylpachymaran(CMP)and postbiotics(PB)derived from Clostridium butyricum and Lactobacillus reuteri on the growth performance and cecal microflora of Kunming mice.Twenty-four male Kunming mice,aged 3 weeks and weighing approximately 10 g,were randomly assigned to four groups with 6 replicates per group and 1 mouse per replicate.The control group received a basal diet,while the experimental groups were fed the basal diet supplemented with 1‰CMP(1‰CMP group),5‰PB(5‰PB group)and 6‰compound(1‰CMP+5‰ PB,6‰ compound group),respectively.The experiment lasted for 13 days.The results showed as follows:1)CMP at concentrations of 2 and 10 mg/mL exhibited scavenging rates of 49.83%and 52.89%for hydroxyl and 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radicals,respectively.Despite this,e-ven at a concentration of 10 mg/mL,CMP demonstrated only limited reducing power and no scavenging activi-ty against superoxide free radicals.2)The postbiotics were produced using a two-step fermentation process,re-sulting in a concentration of 5×108 CFU/mL of viable Clostridium butyricum in the initial step and Lactobacil-lus reuteri in the subsequent step.The composition of CMP,PB,maltodextrin,and light calcium carbonate ex-cipients in the complex was determined to be 25∶5∶19∶1 following the mixing of CMP with PB.3)After a 13-day feeding period,it was observed that the average daily feed intake of mice increased by 8.65%and 11.44%when compared with the 1‰CMP group and 5‰PB group,respectively.Additionally,the average daily gain showed an increase of 12.42%and 8.18%in the same groups,while body weight exhibited a 7.37%and 4.63%increase,respectively.4)The findings from the intestinal microflora sequencing analysis indicate that the compound's mechanism for promoting weight gain in Kunming mice may be associated with a notable ele-vation in the Firmicutes to Bacteroides(F/B)ratio(P＜0.05).Additionally,there was a significant reduction in the abundance of harmful bacteria,such as Helicobacter and Muribaculum intestinale,in the cecal contents of the mice(P＞0.05),whereas the abundance of beneficial bacteria,such as Butyrivibrio hungatei,was sig-nificantly elevated(P＜0.05).Furthermore,multilevel discriminant analysis of species difference(LEfSe)a-nalysis revealed a significant increase in the presence of beneficial bacteria in the gut of the 6‰ compound group.In summary,CMP demonstrates a notable capacity for eliminating hydroxyl free radicals,and the incor-poration of a complex consisting of 6‰ compound(1‰ CMP+5‰ PB)has the potential to enhance the growth performance of Kunming mice through the elevation of the intestinal F/B ratio and enhancement of the intestinal microflora composition.[Chinese Journal of Animal Nutrition,2025,37(3):2003-2018]关键词
羧甲基茯苓多糖/后生元/昆明小鼠/抗氧化性能/生长性能/肠道菌群Key words
carboxymethylpachymaran/postbiotics/Kunming mice/antioxidant properties/growth perform-ance/intestinal microflora分类
农业科技引用本文复制引用
邓清,熊小军,黄河,文少怀,梁嘉敏,李雄英,王跃球,林元山,贺建华,庞一林..羧甲基茯苓多糖、丁酸梭菌和罗伊氏乳杆菌后生元复合物对昆明小鼠生长性能和盲肠菌群结构的影响[J].动物营养学报,2025,37(3):2003-2018,16.基金项目
湖南省高校创新平台开放基金项目(19k045) （19k045）
