大学化学2025，Vol.40Issue(2)：1-10,10.DOI:10.12461/PKU.DXHX202402027
新冠病毒光热/比色双模态定量综合性实验设计
Design of a Comprehensive Experiment for Photothermal/Colorimetric Dual-Mode Quantification of SARS-CoV-2
摘要
Abstract
We have designed a comprehensive experiment for the photothermal/colorimetric dual-mode quantification of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).By developing label nanoprobes with high photothermal conversion efficiency under near-infrared light and integrating them with a self-built small photothermal imager,we have effectively addressed the limitations of commercial colloidal gold test strips,such as low sensitivity and inability to quantify.This method is rapid and user-friendly,requiring only 15 minutes to complete,and enables both naked-eye colorimetric qualification and photothermal accurate quantification.With a detection limit as low as 1.2 pg∙mL-1,this method surpasses commercial colloidal gold test strips by achieving accurate quantitative detection and improving sensitivity by nearly 10,000 times,significantly reducing the detection window period.Teaching practices have shown that this experiment enhances students'comprehensive abilities,expands their academic horizons,and nurtures their scientific research literacy and innovation potential.关键词
Aushell-Fe3O4纳米粒子/比色检测/光热检测/试纸条/新冠病毒Key words
Aushell-Fe3O4 nanoparticles/Colorimetric detection/Photothermal detection/Test strip/SARS-CoV-2分类
化学引用本文复制引用
曾景斌,王文静,赵田雨,郭庚辰,矫春鹏,江永忠,温聪颖..新冠病毒光热/比色双模态定量综合性实验设计[J].大学化学,2025,40(2):1-10,10.基金项目
国家自然科学基金面上项目(21876206,22376218) （21876206,22376218）
山东省自然科学基金面上项目(ZR2020MB064) （ZR2020MB064）
山东省重大基础研究项目(ZR2020ZD13) （ZR2020ZD13）
中国石油大学教学改革项目(CM2022027,CZ2022009) （CM2022027,CZ2022009）
