大学化学2025，Vol.40Issue(2)：121-129,9.DOI:10.12461/PKU.DXHX202409025
暗场显微镜下的纳米颗粒识别与观测细胞膜上的扩散运动
Identification of Nanoparticles and Observation of Diffusion on Cell Membranes Using Darkfield Microscopy:Recommending a Chemical Measurement Experiment
摘要
Abstract
Single-particle tracking represents a significant technological breakthrough for real-time observation of dynamic biological processes,particularly in uncovering mechanisms hidden in static observations.With advancements in optical microscopy,commercial instruments now enable real-time observation of biological processes at the microscopic scale.This experiment explores the size effect of plasmonic nanomaterials using gold and silver nanoparticles as imaging probes.Additionally,live cell imaging experiments are designed to introduce single-particle tracking into undergraduate education by observing the diffusion of gold nanorods on cell membranes as an exemplary experiment in Chemical Metrology and Technology.The experiment is rich in content and engaging,targeting second-and third-year undergraduates who have a foundational understanding of chemistry and chemical biology and are interested in Chemical Metrology and Technology.The goal is to integrate cutting-edge research in single-particle tracking into undergraduate teaching,encouraging independent exploration and stimulating students'interest in scientific research while cultivating their professional skills.关键词
单颗粒示踪/等离激元纳米颗粒/活细胞成像/科教融合/综合化学实验Key words
Single-particle tracking/Plasmonic nanoparticles/Live-cell imaging/Integration of science and education/Integrated chemistry experiments分类
社会科学引用本文复制引用
孙元芳,张然,罗天,张欣,王翊如,黄腾翔,方宁..暗场显微镜下的纳米颗粒识别与观测细胞膜上的扩散运动[J].大学化学,2025,40(2):121-129,9.基金项目
国家自然科学基金(32230063) （32230063）
教育部基础学科拔尖学生培养计划2.0(20222111,20232023) （20222111,20232023）
福建省本科高校教育教学研究重大项目(FBJY20230270) （FBJY20230270）
中央高校基本科研业务费专项(20720220016) （20720220016）
教育部化学"101计划"——化学测量学实验课程建设项目 （）
教育部第三批虚拟教研室建设试点——"101计划"化学测量学实验课程虚拟教研室 （）
