福建农业学报2025，Vol.40Issue(1)：1-9,9.
木薯MebZIP27基因的克隆与原核表达分析
Cloning and Prokaryotic Expressions of Cassava MebZIP27
摘要
Abstract
[Objective]The Class A basic leucine zipper protein(bZIP),which regulates genes carrying ABA-responsive elements associated with the environmental adaptations as well as growth,development,and physiological metabolism of plants,in cassava was studied.[Methods]The Class A bZIP in cassava,MebZIP27,was cloned using RT-PCR technology to determine the functions of the gene transcription factors.Bioinformatic methods were applied to analyze properties of the gene and protein,fluorescence quantitative PCR employed to examine spatiotemporal expressions and responses to stress,and prokaryotic expression used to produce MebZIP27 protein.[Results]MebZIP27 was in the 5th chromosome of cassava with a coding region of 1251 bp and characteristic bZIP domain features.It had the highest homology with the Class A bZIP sequences from Hevea rubber at 80.00%and Jatropha curcas at 85.41%,as a member of the A-bZIP subfamily.Located in nucleus,the gene had the highest expression found in the roots.Treatments of mannitol,NaCl or low temperature did not cause significant induction,but ABA and H2O2 did on it.Via the prokaryotic expression,MebZIP27 could be produced and purified using glutathione agarose bead separation techniques.[Conclusion]Located in nucleus and belonged to the Class A bZIP gene family,MebZIP27 expressed most significantly in the storage roots of a cassava plant and by an ABA or H2O2 induction.关键词
木薯/bZIPs/非生物胁迫/原核表达Key words
cassava/bZIP/abiotic stress/prokaryotic expression分类
农业科学引用本文复制引用
曾力旺,胡伟,张小燕,林墁,杨静琳,林素妍,潘子墨,雷鑫芳,吴春来,曾坚..木薯MebZIP27基因的克隆与原核表达分析[J].福建农业学报,2025,40(1):1-9,9.基金项目
广东省基础与应用基础研究基金项目(2025A1515010531、2023A1515010336) （2025A1515010531、2023A1515010336）
广东省普通高校重点领域专项(2024ZDZX4018、2022ZDZX4047) （2024ZDZX4018、2022ZDZX4047）
韶关学院重点项目(SZ2022KJ05) （SZ2022KJ05）
韶关学院大学生创新创业训练计划项目(Sycxcy2024176) （Sycxcy2024176）
海南省重点研发计划项目(ZDYF2022XDNY259) （ZDYF2022XDNY259）
中央级公益性科研院所基本科研业务费专项(1630052022017、1630052023016) （1630052022017、1630052023016）
