福建农业学报2025，Vol.40Issue(1)：71-80,10.
豇豆枯萎病病原种类鉴定及防控药剂筛选
Pathogen Identification and Effective Fungicide for Cowpea Wilt Disease
摘要
Abstract
[Objective]Pathogens causing the cowpea wilt disease and an effective control agent were identified.[Method]Field surveys and statistical analyses were conducted to assess the prevalence of the wilt disease on cowpea plants at various growth stages.Infected plants were collected for pathogen isolation and purification in the laboratory.Suspected microorganisms were examined for morphological characteristics,analyzed for pathogenicity,and identified based on molecular determination.Mycelial growth rate as well as inhibitory effects on the potential pathogenic fungi and oomycetes under treatments of 10 fungicides were determined.[Result]Preliminary,135 strains of microbes and 128 of oomycetes were isolated and purified.They included fungi like Fusarium oxysporum and Nigrospora osmanthi and oomycetes like Phytophthora vignae and P.nicotianae that displayed the disease symptoms on cowpeas.The mycelial growth of F.oxysporum showed varying degrees of sensitivity toward the 10 fungicides.Prochloraz had the highest inhibitory effect of an EC50 of 0.086 μg·mL-1 followed by difenoconazole with an EC50 of 0.241 μg·mL-1 and carbendazim with an EC50 of 0.944 μg·mL-1,while copper oxychloride least effective with a dismal EC50 of 14 719.994 μg·mL-1.The inhibition rates of cymoxanil-azoxystrobin were 55.32%against F.oxysporum and 100%against P.vignae and P.nicotianae.It appeared to be the fungicide most effective for controlling,preventing,and treating the disease on cowpeas.[Conclusion]The pathogenic species of cowpea wilt were clarified,and it was first report demonstrating that the pathogenic fungus N.osmanthi and the pathogenic oomycetes P.nicotianae could infect cowpea and cause cowpea disease.Effective control agents with significant inhibitory effects against pathogens were successfully screened,providing a scientific basis for the integrated management of cowpea wilt disease.关键词
豇豆/枯萎病/病原真菌/病原卵菌/防控药剂Key words
cowpea/wilt disease/pathogenic fungi/pathogenic oomycetes/control agents分类
植物保护学引用本文复制引用
张馨予,洪丹露,冯婉珍,陈庆河..豇豆枯萎病病原种类鉴定及防控药剂筛选[J].福建农业学报,2025,40(1):71-80,10.基金项目
国家重点研发计划项目(2024YFD1400100) （2024YFD1400100）
海南省重点研发项目(ZDYF2024XDNY250-3) （ZDYF2024XDNY250-3）
