福建农业学报2025，Vol.40Issue(1)：81-90,10.
莫海威芽孢杆菌JK-18的全基因组测序及拮抗相关基因簇的分析
Genome Sequencing and Antimicrobial Metabolite Synthesis Genes of Bacillus mojavensis JK-18
摘要
Abstract
[Objective]Sequence and annotation of Bacillus mojavensis JK-18 genome were obtained,and gene clusters related to the synthesis of antimicrobial secondary metabolites in the bacterium identified for the development of a biocontrol agent for walnut tree blight.[Method]The whole genome of B.mojavensis JK-18 isolated from the rhizosphere soil of walnut trees was sequenced using the PacBio technology.The characteristics,functions,and classification of the gene and secondary metabolites secreted by the bacterium were analyzed.[Results]The genome was a closed circular DNA with a size of 4 166 746 bp containing 4 138 protein-coding genes and 4 kinds of CRISPR.It had 5 844,2 299,3 257,183,and 7 functional genes annotated in GO,KEGG,EGGNOG,CAZY,and CARD databases,respectively.Predicted by antiSMASH,the genes related to the synthesis of secondary metabolites including the antimicrobial substances like polyketide compounds(e.g.,Lichenysin,Macrobrevin and Aurantinin),peptidolipids(e.g.,Surfactin,Bacillomycin,Plipastatin and Fengycin),and macrolides(e.g.,Dumulmycin and Dactylocycline A).[Conclusion]Identification of the genes in B.mojavensis JK-18 that related to the syntheses of secondary metabolites with an antimicrobial property would aid further study leading to the development of bioagents for blight prevention and control on walnut trees.关键词
核桃/莫海威芽孢杆菌/全基因组测序/合成基因簇Key words
walnut/Bacillus mojavensis/whole-genome sequencing/synthetic gene cluster分类
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王瀚,王攀,田凤鸣,卓平清,马静静..莫海威芽孢杆菌JK-18的全基因组测序及拮抗相关基因簇的分析[J].福建农业学报,2025,40(1):81-90,10.基金项目
陇南市科技计划项目(2022-S.JH-12) （2022-S.JH-12）
甘肃省科学技术协会 2023 年创新驱动项目(GXH20230817-8) （GXH20230817-8）
