临床与病理杂志2024，Vol.44Issue(12)：1628-1637,10.DOI:10.11817/j.issn.2095-6959.2024.240794
血清C/EBP同源蛋白、葡萄糖调节蛋白78及成纤维细胞生长因子21与轻度至中重度老年性耳聋的相关性
Correlation of serum CHOP,GRP78,and FGF21 with mild to moderate age-related hearing loss
周楠 1李世宝 2袁金涛 1王采集 3蒋雯 3赵泽祺 3刘稳 3李轩毅 3乔月华3
作者信息
- 1. 徐州医科大学第一临床医学院,江苏 徐州 221004||徐州医科大学附属医院耳鼻咽喉科,江苏 徐州 221002
- 2. 徐州医科大学附属医院检验科,江苏 徐州 221002
- 3. 徐州医科大学附属医院耳鼻咽喉科,江苏 徐州 221002
- 折叠
摘要
Abstract
Objective:In recent years,studies on endoplasmic reticulum stress(ERS)have increased,revealing its involvement in a variety of age-related diseases.Some scholars have confirmed a correlation between age-related hearing loss(ARHL)and ERS in animal and cell models.This study aims to explore the clinical correlation between serum C/EBP homologous protein(CHOP),glucose-regulated protein 78(GRP78),and fibroblast growth factor 21(FGF21)with mild to moderate ARHL.
Methods:A total of 50 patients with mild to moderate ARHL were enrolled at the Affiliated Hospital of Xuzhou Medical University,serving as a hearing loss group,and 30 healthy individuals undergoing physical examination during the same period were included as a control group.All participants underwent clinical data collection and pure tone audiometry.According to audiometry results,subjects were divided into a normal(n=30,37.5%),a mild(n=29,36.25%),a moderate(n=14,17.5%),and a moderate-to-severe(n=7,8.75%)hearing loss groups.Serum CHOP,GRP78,and FGF21 levels were measured using enzyme-linked immunosorbent assay(ELISA).Spearman correlation analysis was used to assess the relationship between these serum markers and ARHL.Multivariate logistic regression identified influencing factors,and receiver operating characteristic(ROC)curve was used to evaluate the predictive value of the markers.
Results:Compared to the control group,serum levels of CHOP,GRP78,and FGF21 were significantly elevated in the hearing loss group(all P<0.05).The proportion of patients with smoking history and poor sleep quality was also higher in the hearing loss group than that in the control group(Both P<0.05).Spearman analysis showed a positive correlation between CHOP levels and hearing loss severity(P<0.05).Logistic regression showed that sleep quality was a protective factor for ARHL(P=0.030),and GRP78 was a risk factor for ARHL(P=0.031).ROC analysis showed:CHOP had an area under the curve(AUC)of 0.677(sensitivity 68.0%,specificity 63.3%);GRP78 had an AUC of 0.812(sensitivity 66.0%,specificity 96.7%);FGF21 had an AUC of 0.828(sensitivity 62.0%,specificity 96.7%).
Conclusion:Patients with mild to moderate ARHL may exhibit ERS dysregulation.Serum CHOP,GRP78,and FGF21 levels are correlated with the occurrence of ARHL,and FGF21 may provide a new idea for the early prevention of ARHL.关键词
年龄相关性听力损失/内质网应激/C/EBP同源蛋白/葡萄糖调节蛋白78/成纤维细胞生长因子21/未折叠蛋白反应Key words
age-related hearing loss/endoplasmic reticulum stress/C/EBP homologous protein/glucose-regulating protein 78/fibroblast growth factor 21/unfolded protein reaction引用本文复制引用
周楠,李世宝,袁金涛,王采集,蒋雯,赵泽祺,刘稳,李轩毅,乔月华..血清C/EBP同源蛋白、葡萄糖调节蛋白78及成纤维细胞生长因子21与轻度至中重度老年性耳聋的相关性[J].临床与病理杂志,2024,44(12):1628-1637,10.
