临床与病理杂志2024，Vol.44Issue(12)：1645-1653,9.DOI:10.11817/j.issn.2095-6959.2024.240868
儿童非酒精性单纯性脂肪肝肠道菌群特征
Gut microbiota characteristics in children with nonalcoholic fatty liver
摘要
Abstract
Objective:Gut microbiota dysbiosis is a key factor in the pathogenesis of nonalcoholic fatty liver(NAFL)in children,influencing disease onset and progression through mechanisms such as metabolic pathway disruption,inflammation,and damage to the intestinal barrier.This study aims to explore the gut microbiota composition and characteristic taxa in children with NAFL. Methods:From January to April 2024,children diagnosed with NAFL at the First Affiliated Hospital of Henan University of Chinese Medicine were enrolled as a NAFL group,while an equal number of healthy volunteers served as a control group.16S rRNA sequencing was used to analyze fecal samples from both groups for diversity,structural differences,and characteristics bacterial genera.Functional predictions of gut microbiota were also performed. Results:The Chao1 index in the NAFL group was significantly lower than that in the healthy group(P<0.05),indicating reduced microbial richness.The Shannon and Simpson indices were also lower in the NAFL group,but the differences were not statistically significant(P>0.05).Compared with the healthy group,the relative abundances of Firmicutes,Proteobacteria,and Actinobacteria were higher in the NAFL group,though not statistically significant(all P>0.05),whereas the abundance of Bacteroidetes was significantly lower(P<0.05).Functional prediction revealed enhanced pathways in the NAFL group,including arginine biosynthesis,the tricarboxylic acid cycle,D-galactose degradation,and uridine diphosphate-N-acetyl-D-glucosamine biosynthesis.In contrast,pathways related to cis-11-eicosenoic acid biosynthesis,and fatty acid elongation,and mannose biosynthesis were relatively weakened. Conclusion:Children with NAFL exhibit significant alterations in gut microbiota composition and abundance.Actinobacteria may serve as a potential marker.These changes may contribute to disease development by affecting multiple metabolic pathways.关键词
非酒精性单纯性脂肪肝/肠道菌群/16S rRNA/儿童及青少年/肥胖/超重Key words
nonalcoholic fatty liver/gut microbiota/16S rRNA/children and adolescents/obesity/overweight引用本文复制引用
孟璐,郑宏,徐炎,邱建利,管志伟,李银丽,渠怡彤..儿童非酒精性单纯性脂肪肝肠道菌群特征[J].临床与病理杂志,2024,44(12):1645-1653,9.基金项目
国家自然科学基金(81804142,82205190),河南省自然科学基金(232300420057),中国博士后科学基金(2020M670026ZX,2023M731027),河南省博士后项目(201901029,HN2022096).This work was supported by the National Natural Science Foundation(81804142,82205190),the Natural Science Foundation of Henan Province(232300420057),China Postdoctoral Science Foundation(2020M670026ZX,2023M731027),and Henan Provincial Postdoctoral Science Foundation(201901029,HN2022096),China. （81804142,82205190）
