广西植物2025，Vol.45Issue(3)：438-449,12.DOI:10.11931/guihaia.gxzw202502016
基于CRISPR/Cas9的猕猴桃性状改良:精准育种策略与挑战
Kiwifruit trait improvement via CRISPR/Cas9:Precision breeding strategies and challenges
摘要
Abstract
The rapid advancement of gene editing technologies has revolutionized precision breeding in kiwifruit(Actinidia spp.).This review systematically summarizes the multidimensional applications,strategies,and challenges of CRISPR/Cas9 technology in kiwifruit trait improvement.Leveraging high-quality genomic resources,such as the telomere-to-telomere gapless reference genome of A.chinensis,and efficient genetic transformation systems like marker-free Agrobacterium-mediated methods,researchers have achieved breakthroughs in fruit quality,disease resistance,and plant architecture regulation.Key advancements include:targeted editing of AcNAC1,bZIP and MYB/bHLH complexes to reduce citrate content,enhance vitamin C biosynthesis,and optimize anthocyanin accumulation;a host-pathogen dual-targeting strategy that strengthens the AcCBL3-mediated calcium oxalate barrier and disrupts the hopAI1 virulence gene in pathogens,significantly improving disease resistance;and knockout of CEN-like and AcFLC-like genes to develop compact plant architecture and non-cold-independent budbreak germplasms.Postharvest studies have elucidated synergistic regulatory networks between ethylene signaling and cell wall hydrolases,offering molecular targets for shelf-life extension.Despite challenges such as polyploid editing complexity and transgenic regulatory controversies,the integration of multi-omics and synthetic biology tools is advancing kiwifruit breeding from single-gene manipulation to metabolic pathway reprogramming.With global regulatory relaxation for foreign DNA-free edited varieties,CRISPR/Cas9-based molecular design breeding will usher in an important opportunity period of industrial development.In addition,this review further outlines technical optimization pathways and future research priorities,providing a theoretical framework for accelerating the breakthrough breeding of kiwifruit cultivars.关键词
猕猴桃育种/CRISPR/Cas9基因编辑/全基因组测序/分子设计育种/果实品质改良Key words
kiwifruit breeding/CRISPR/Cas9 gene editing/whole genome sequencing/molecular design breeding/fruit quality improvement分类
生物学引用本文复制引用
朱荣香,蒋桥生,王发明,刘玉红,李洁维,叶开玉,刘翠霞,夏黎明,龚弘娟,齐贝贝,高建有..基于CRISPR/Cas9的猕猴桃性状改良:精准育种策略与挑战[J].广西植物,2025,45(3):438-449,12.基金项目
广西科技重大专项(桂科AA23023008) （桂科AA23023008）
国家现代农业产业技术体系"广西落叶果树产业创新团队"项目(nycytxgxcxtd-2023-13-01) （nycytxgxcxtd-2023-13-01）
广西植物研究所基本业务费项目(桂植业24007) （桂植业24007）
广西植物功能物质与资源持续利用重点实验室项目(ZRJJ2023-3). （ZRJJ2023-3）
