广西植物2025，Vol.45Issue(3)：517-526,10.DOI:10.11931/guihaia.gxzw202310044
地枫皮入血成分抗类风湿性关节炎作用研究
Anti-rheumatoid arthritis effect of entering bloodstream components from Illicium difengpi
摘要
Abstract
To search the compounds with anti-rheumatoid arthritis(anti-RA)efficacy from Illicium difengpi,the components that can enter bloodstream were identified by liquid chromatography-tandem mass spectrometry(LC-MS/MS),and then their anti-RA activities were evaluated by testing their effects on symptoms,arthritis indexes,pathological changes of knee joints,and contents of MCP-1,IL-6 and TNF-α in serum of collagen-induced arthritis mice.The interactions between the anti-RA active compounds and TLR4/NF-κB pathway were studied by molecular docking.The results were as follows:(1)After absorbance,isodunnianol and magnolol of Ⅰ.difengpi could enter bloodstream.(2)Isodunnianol could alleviate symptoms including limbs congestion,redness and swelling,reduce arthritis index score of limbs,hinder pathological process of knee joints tissue,and decrease contents of MCP-1,IL-6 and TNF-α in serum of collagen-induced arthritis mice.(3)Isodunnianol could bind to key proteins of TLR4/NF-κB pathway,including TLR4,IκBα and NF-κB p65.In conclusion,isodunnianol can reach the joint tissue through the bloodstream after being absorbed and can possibly alleviate the pathology of RA through regulating TLR4/NF-κB pathway.Isodunnianol is one of anti-RA active components of Ⅰ.difengpi.关键词
地枫皮/异红花八角醇/类风湿性关节炎/入血/液相色谱-串联质谱法/TLR4/NF-κB通路Key words
Illicium difengpi/isodunnianol/rheumatoid arthritis(RA)/enter bloodstream/LC-MS/MS/TLR4/NF-κB pathway分类
农业科技引用本文复制引用
李连春,符毓夏,谭兰芳,宁德生,潘争红..地枫皮入血成分抗类风湿性关节炎作用研究[J].广西植物,2025,45(3):517-526,10.基金项目
国家自然科学基金(31960091) （31960091）
广西自然科学基金(2019GXNSFAA245060) （2019GXNSFAA245060）
桂林市创新平台和人才计划(20210102-3). （20210102-3）
