广西植物2025，Vol.45Issue(3)：527-541,15.DOI:10.11931/guihaia.gxzw202308050
六堡茶群体种叶绿体基因组捕获历史与遗传多样性研究
Chloroplast genome capture history and genetic diversity of Camellia sinensis var.sinensis'Liupao'
摘要
Abstract
Camellia sinensis var.sinensis'Liupao'is the main raw material for making Liupao tea.To explore the genetic background of C.sinensis var.sinensis'Liupao',especially its phylogenetic position and evolutionary history,27 individuals of C.sinensis var.sinensis'Liupao'and five individuals of C.costata were sampled for genome skimming and transcriptomic sequencing.The phylogenetic relationship,the divergence evolution time,and the genetic diversity of C.sinensis var.sinensis'Liupao'were analyzed.The results were as follows:(1)On the chloroplast tree,samples of C.sinensis var.sinensis'Liupao'were divided into two distantly-related groups,one was nested within a clade mainly consisting of C.sinensis,being interspersed among other cultivated C.sinensis,while the other group formed as a well supported lineage that was most closely-related to and nested within C.costata;on the nuclear gene tree,however,all the 27 samples of C.sinensis var.sinensis'Liupao'formed a clade with other C.sinensis as well as some other Camellia species with generally unresolved relationships among them.Despite the lack of resolution in this clade,it was definitely far separated from C.costata;the cytonuclear phylogenetic conflict suggested once ancient introgression hybridization of C.costata with the ancestor of some C.sinensis var.sinensis'Liupao'individuals so that the latter captured the chloroplast genome of the former.(2)The time estimate further indicated that the introgression hybridization event occurred in the Quaternary period,ca.1.55 million years ago,long before the history of tea cultivation and production by humans.(3)In addition,both the chloroplast genomes and nuclear genes revealed that the C.sinensis var.sinensis'Liupao'had high haplotype diversity,possessing high evolutionary potential.This study provides the references for the germplasm protection,variety breeding,development and utilization of C.sinensis var.sinensis'Liupao'.关键词
六堡茶群体种/叶绿体基因组/转录组/叶绿体基因组捕获/杂交/遗传多样性Key words
Camellia sinensis var.sinensis'Liupao'/chloroplast genomes/transcriptomes/chloroplast genome capture/hybridization/genetic diversity分类
农业科技引用本文复制引用
姚淑婷,张强,苏敏,吴雨婷,庞月兰,梁燕妮,蔡爱华,覃信梅..六堡茶群体种叶绿体基因组捕获历史与遗传多样性研究[J].广西植物,2025,45(3):527-541,15.基金项目
广西创新驱动发展专项(桂科AA20302018-1) （桂科AA20302018-1）
广西自然科学基金(2023GXNSFAA026221) （2023GXNSFAA026221）
广西喀斯特植物保育与恢复生态学重点实验室项目(22-035-26). （22-035-26）
