广西植物2025，Vol.45Issue(3)：585-605,21.DOI:10.11931/guihaia.gxzw202409042
采用液质联用技术定性分析松叶鸡蛋参根的化学成分
Qualitative analysis of chemical constituents of Codonopsis convolvulacea var.pinifolia roots by using LC-MS
摘要
Abstract
To clarify the chemical constituents of the Tibetan medicine Codonopsis Convolvulaceae Radix(the roots of Codonopsis convolvulacea var.pinifolia)by ultra high performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UHPLC-Q-TOF-MS/MS),Waters ACQUITY UPLC BEH C18(1.7 μm,2.1 mm × 150 mm)chromatographic column was used.The mobile phase was 0.1 mmol·L-1 ammonium acetate aqueous solution and acetonitrile.The flow rate was 0.3 mL·min-1,the injection volume was 3 μL,and the column temperature was 40℃.Electrospray ionization(ESI)source was used for mass spectrometry,and the detection was conducted in negative ion mode.The mass spectrometry scanning range was m/z 100-1 800(MS)and m/z 50-1 800(MS/MS).The molecular formula of a compound was deduced based on quasi-molecular ions in its first-order mass spectra,and its structural fragments and possible molecular structure were elucidated by analyzing the characteristic fragment ions in its second-order mass spectra.Finally,its structure was indentified by comparison with reference material and literature retrieval.The results were as follows:(1)Totally 56 compounds were tentatively identified from the roots of C.convolvulacea var.pinifolia for the first time,including 6 nitrogen-containing compounds,6 phenylpropanoids,22 lignans,2 flavonoids,8 organic acids,and 12 glucosides or other compounds.Among them,11 compounds were unequivocally identified by comparison with reference compounds.(2)Lignans and phenylpropanoids were firstly found to be the main constituents of C.convolvulacea var.pinifolia roots,and their fragmentation pathways were deduced.In this study,the application of UHPLC-Q-TOF-MS/MS can quickly and efficiently elucidate chemical constituents of C.convolvulacea var.pinifolia roots.It provides a chemical basis for further studies of quality standard,in vivo process and pharmacodynamic substances of Codonopsis Convolvulaceae Radix.关键词
松叶鸡蛋参/UHPLC-Q-TOF-MS/MS/化学成分/结构解析/裂解途径Key words
Codonopsis convolvulacea var.pinifolia/UHPLC-Q-TOF-MS/MS/chemical constituents/structural elucidation/fragmentation pathway分类
农业科技引用本文复制引用
赵文慧,窦志扬,强巴,吾坚,白玛卓玛,刘广学,蔡少青,徐风..采用液质联用技术定性分析松叶鸡蛋参根的化学成分[J].广西植物,2025,45(3):585-605,21.基金项目
西藏自治区2024年度科技计划科技重大专项 （）
林芝市2022年度"区域科技协同创新"专项. （）
