海南医科大学学报2025，Vol.31Issue(6)：401-407,7.DOI:10.13210/j.cnki.jhmu.20240823.002
类鼻疽伯克霍尔德菌非编码RNA BprsB的生物学功能初步评价
Preliminary evaluation of the biological function of non-coding RNA BprsB in Burkholderia pseudomallei
摘要
Abstract
Objective:To investigate the effects of Hfq related sRNA(BprsB)on the growth rate,motility,biofilm forma-tion,and drug resistance of Burkholderia pseudomallei.Method:Using homologous recombination technology,BprsB of Burk-holderia pseudomallei dependent on Hfq protein was used as the target to construct a BprsB mutantt strain(ΔBprsB);The wild-type Burkholderia pseudomallei strain(HNBP001)was used as the control group,and the ΔBprsB was used as the experi-mental group;The bacterial growth curve was measured by a multimode reader to evaluate changes in growth rate;Using point plate culture method and measuring the diameter of the colony to evaluate motility;The formation of biofilm was measured by crystal violet staining method;Finally,the sensitivity of bacteria to antibiotics is determined through MIC experiments.Result:The growth rate of ΔBprsB was lower than that of HNBP001;Compared with HNBP001,the population diameter of ΔBprsB was smaller(P<0.000 1);The biofilm formation of ΔBprsB was less than that of HNBP001(P<0.000 1);Compared with HN-BP001,the ΔBprsB was more sensitive to quinolone antibiotics(Levofloxacin,Norfloxacin,Ciprofloxacin)and β-lactam antibiot-ics(Aztreonam).Conclusion:sRNA BprsB promotes the growth rate,motility,biofilm formation,and drug resistance of B.pseu-domallei,laying a solid foundation for further research on the role of sRNA BprsB in the pathogenesis of B.pseudomallei.关键词
类鼻疽伯克霍尔德菌/sRNA/基因敲除/生物学功能Key words
Burkholderia pseudomallei/sRNA/Gene knockout/Biological function分类
基础医学引用本文复制引用
谭君,李雪苗,罗妮妮,王彦双,陈垂这,邓泽芳,罗江慧,方俊德,夏乾峰..类鼻疽伯克霍尔德菌非编码RNA BprsB的生物学功能初步评价[J].海南医科大学学报,2025,31(6):401-407,7.基金项目
This study was supported by National Natural Science Foundation of China(81960002) 国家自然科学基金资助项目(81960002) （81960002）
