基于网络药理学及实验验证探讨吴茱萸汤减轻脓毒症大鼠肺损伤的作用机制
Exploring the mechanism of Wuzhuyu decoction in reducing lung injury in sepsis rats based on network pharmacology and experimental validation
摘要
Abstract
Objective:To pharmacology and experimental verification explore the possible mechanism of Wuzhuyu decoction in reducing lung injury in septic rats based on network.Methods:TCMSP and ETCM databases were used to screen and deter-mine the active ingredients in Wuzhuyu decoction.Effective targets were selected using the Uniport database,and disease targets for sepsis-induced lung injury were obtained using GeneCards and OMIM databases.The intersection targets of the two were se-lected using the online Venny platform.A PPI network was constructed using the String platform,and core targets were screened using Cytoscape 3.7.1 software.The DAVID database was utilized to analyze GO and KEGG pathway and a"Drug-Disease-Path-way"network diagram was constructed.The experimental validation section used the CLP method to establish a rat model of sep-sis-induced lung injury.After drug intervention,the survival rate and pathological changes in lung tissue of each group of rats were observed,and the wet/dry weight ratio of lung tissue was compared.The white blood cell count,levels of related inflammatory factors in BALF and expression of NF-κB protein in lung tissue were detected.Results:This research screened 159 targets of Wu-zhuyu decoction for the cure of sepsis lung injury,including TNF-α,IL-1β,IL-6,etc,involving 20 key pathways including NF-κB signaling pathway.The experimental verification results showed that the survival rates of rats in each treatment group were im-proved compared to the model group(P<0.05),while the medium dose group of Wuzhuyu decoction showed the most significant improvement compared to the other treatment groups(P<0.05).The wet/dry ratio of lung tissue in the model group was higher than the sham surgery group(P<0.05),while each treatment group showed a decrease compared to the model group(P<0.05).Among them,the medium dose group of Wuzhuyu decoction showed the most significant decrease compared to the other treat-ment groups(P<0.05).The model group showed an increase in white blood cell count,TNF-α,IL-1β and IL-6 levels in BALF compared to the sham surgery group(all P<0.05),while each treatment group showed a decrease compared to the model group(all P<0.05).Among them,the medium dose group of Wuzhuyu decoction showed the most significant decrease compared to the other treatment groups(P<0.05).The expression of NF-κB p65 protein in the lung tissue of rats in the model group was upregu-lated compared to the sham surgery group(P<0.05),while each treatment group was downregulated compared to the model group(P<0.05).Among them,the medium dose group of Wuzhuyu decoction had the most significant downregulation compared to the other treatment groups(P<0.05).Conclusion:Wuzhuyu decoction can reduce the levels of TNF-α,IL-1β and IL-6,down-regulate the expression of NF-κB protein,and alleviate lung injury by inhibiting inflammation in septic rats.This mechanism may be related to the NF-κB signaling pathway.关键词
吴茱萸汤/脓毒症/肺损伤/抗炎/网络药理学Key words
Wuzhuyu decoction/Sepsis/Lung injury/Inhibit inflammation/Network pharmacology分类
医药卫生引用本文复制引用
金颂峰,于雪峰,尹江涛,邸静鑫,关玉娟,丁晓欢,甘雨,崔英海,张继伟,陈美娟,林宇康..基于网络药理学及实验验证探讨吴茱萸汤减轻脓毒症大鼠肺损伤的作用机制[J].海南医科大学学报,2025,31(6):433-442,10.基金项目
This study was supported by the National Key Research and Development Program(2018YFC1707410) （2018YFC1707410）
Regional Traditional Chinese Medicine Diagnosis and Treatment Center Project of the State Administration of Traditional Chinese Medicine(Traditional Chinese Medicine Lung Disease Specialty) （Traditional Chinese Medicine Lung Disease Specialty）
Collaborative Innovation and Transformation Project for High Quality Development of Traditional Chinese Medicine by the State Administration of Traditional Chinese Medicine(CXZH04) （CXZH04）
Natural Science Foundation of Liaoning Province[Liaokefa(2022)No.32] （2022）
Construction Project of Yu Xuefeng Liaoning Provincial Famous Traditional Chinese Medicine Inheritance Studio[Liaoning Traditional Chinese Medicine Development(2022)No.1] （2022）
Doctoral Initiation Fund of Liaoning Provincial Department of Science and Technology(2022-BS-068) （2022-BS-068）
Shenyang Science and Technology Plan Project Public Health Research and Development Special Project(241002,240997) （241002,240997）
The"Nursery Project"of the Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine[Liaozhong R&D(2023)No.5] 国家重点研发计划(2018YFC1707410) （2023）
国家中医药管理局区域中医诊疗中心项目(中医肺病专业) （中医肺病专业）
国家中医药管理局中医药高质量发展协同创新转化工程项目(CXZH04) （CXZH04）
辽宁省自然科学基金计划项目[辽科发(2022)32号] （2022）
于雪峰辽宁省名中医传承工作室建设项目[辽中医药发(2022)1号] （2022）
辽宁省科技厅博士启动基金(2022-BS-068) （2022-BS-068）
沈阳市科学技术计划项目公共卫生研发专项(241002、240997) （241002、240997）
辽宁中医药大学附属第二医院"育苗工程"项目[辽中研发(2023)5号] （2023）
